Sample overview

Sample ID: Sample_1025
Biosample name: Macrophage
Biosample type: Primary Cell
Tissue type: Peripheral blood
Cancer type: Normal
Region number: 14930
Length: 5587.141 kb
GEO/SRA ID: SRR4450160
Mean insert size: 103.708
Standard deviation: 68.019
TSS enrichment score: 6.51512
FRiP: 0.02732
Diasgnostic plot
The percentages of read pairs in FR, RF, and TANDEM orientations.


Accessible chromatin region of Macrophage

Use these accessible chromatin regions to analyze or download

TF footprint of Macrophage


Peak annotation visualization of Macrophage

Plot A

Plot B

Plot A: a pie chart of annotated genomic features.
Note A: the proportion of annotating genomic region (Promoter, 5'UTR, 3'UTR, Exon, Intron, Downstream and Intergenic).
Plot B: a heatmap of peaks binding to TSS region.
Note B: the profile of ATAC peaks binding to TSS region (+/- 1kb).

Software and parameters used

Read mapping: bowtie2(v2.2.5) -p 10 -X 2000 -x -S
Duplicates Removing: picard MarkDuplicates REMOVE_DUPLICATES=true ASSUME_SORTED=true
Peak calling: macs2(2.1.2) --broad--SPMR --nomodel --extsize 200 -q 0.01
Trimming the adapter: trim_galore --phred33 --stringency 3 --length 20