| Sample ID: | Sample_0446 |
| Biosample name: | U2OS |
| Biosample type: | Cell Line |
| Tissue type: | Bone |
| Cancer type: | Osteosarcoma |
| Region number: | 2771 |
| Length: | 1919.4 kb |
| GEO/SRA ID: | SRR7537486 |
| Mean insert size: | 111.315 |
| Standard deviation: | 88.488 |
| TSS enrichment score: | 4.57392 |
| FRiP: | 0.00398 |
| Diasgnostic plot | |
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| Plot A: a pie chart of annotated genomic features. |
| Note A: the proportion of annotating genomic region (Promoter, 5'UTR, 3'UTR, Exon, Intron, Downstream and Intergenic). |
| Plot B: a heatmap of peaks binding to TSS region. |
| Note B: the profile of ATAC peaks binding to TSS region (+/- 1kb). |
| Read mapping: | bowtie2(v2.2.5) -p 10 -X 2000 -x -S |
| Duplicates Removing: | picard MarkDuplicates REMOVE_DUPLICATES=true ASSUME_SORTED=true |
| Peak calling: | macs2(2.1.2) --broad--SPMR --nomodel --extsize 200 -q 0.01 |
| Trimming the adapter: | trim_galore --phred33 --stringency 3 --length 20 |
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