Sample overview

Sample ID: Sample_0060
Biosample name: Forebrain neurons
Biosample type: Induced pluripotent stem cell line
Tissue type: Forebrain
Cancer type: Normal
Region number: 35076
Length: 20145.202 kb
GEO/SRA ID: SRR5417024
Mean insert size: 154.849
Standard deviation: 138.641
TSS enrichment score: 11.44050
FRiP: 0.10743
Diasgnostic plot
The percentages of read pairs in FR, RF, and TANDEM orientations.


Accessible chromatin region of Forebrain neurons

Use these accessible chromatin regions to analyze or download

TF footprint of Forebrain neurons


Peak annotation visualization of Forebrain neurons

Plot A

Plot B

Plot A: a pie chart of annotated genomic features.
Note A: the proportion of annotating genomic region (Promoter, 5'UTR, 3'UTR, Exon, Intron, Downstream and Intergenic).
Plot B: a heatmap of peaks binding to TSS region.
Note B: the profile of ATAC peaks binding to TSS region (+/- 1kb).

Software and parameters used

Read mapping: bowtie2(v2.2.5) -p 10 -X 2000 -x -S
Duplicates Removing: picard MarkDuplicates REMOVE_DUPLICATES=true ASSUME_SORTED=true
Peak calling: macs2(2.1.2) --broad--SPMR --nomodel --extsize 200 -q 0.01
Trimming the adapter: trim_galore --phred33 --stringency 3 --length 20