About Enhancer
| Enhancer ID: | E_01_0394 |
| Species: | human |
| Position : | chr8:19898949-19900949   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 29928592 |
| Enhancer experiment: | RT-qPCR,oil red O staining,ALP activity assay,cell matrix ALP staining, alizarin red S staining, |
| Enhancer experiment description: | TBBPA did not influence osteoblast differentiation, but TCDD suppressed ALP staining and activity, calcium deposition, and osteoblast-related mRNA levels. In a mixture of TBBPA and TCDD, TBBPA inhibited TCDD suppression of adipocyte and osteoblast differentiation in a dose-dependent manner. Interestingly, we observed lipid droplets in TBBPA-treated cells differentiated into osteoblasts. These results suggest that TBBPA and TCDD disrupted differentiation into adipocytes and osteoblasts and contributes to a more complete toxicological understanding of exposure to these chemical substances. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | TBBPA did not influence osteoblast differentiation, but TCDD suppressed ALP staining and activity, calcium deposition, and osteoblast-related mRNA levels. In a mixture of TBBPA and TCDD, TBBPA inhibited TCDD suppression of adipocyte and osteoblast differentiation in a dose-dependent manner. Interestingly, we observed lipid droplets in TBBPA-treated cells differentiated into osteoblasts. These results suggest that TBBPA and TCDD disrupted differentiation into adipocytes and osteoblasts and contributes to a more complete toxicological understanding of exposure to these chemical substances. |
About TF
| TF name : | LPL |
| TF experiment: | RT-qPCR,oil red O staining,ALP activity assay,cell matrix ALP staining, alizarin red S staining, |
| TF experiment description: | TBBPA did not influence osteoblast differentiation, but TCDD suppressed ALP staining and activity, calcium deposition, and osteoblast-related mRNA levels. In a mixture of TBBPA and TCDD, TBBPA inhibited TCDD suppression of adipocyte and osteoblast differentiation in a dose-dependent manner. Interestingly, we observed lipid droplets in TBBPA-treated cells differentiated into osteoblasts. These results suggest that TBBPA and TCDD disrupted differentiation into adipocytes and osteoblasts and contributes to a more complete toxicological understanding of exposure to these chemical substances. |
About Function
| Enhancer function : | TBBPA did not influence osteoblast differentiation, but TCDD suppressed ALP staining and activity, calcium deposition, and osteoblast-related mRNA levels. In a mixture of TBBPA and TCDD, TBBPA inhibited TCDD suppression of adipocyte and osteoblast differentiation in a dose-dependent manner. Interestingly, we observed lipid droplets in TBBPA-treated cells differentiated into osteoblasts. These results suggest that TBBPA and TCDD disrupted differentiation into adipocytes and osteoblasts and contributes to a more complete toxicological understanding of exposure to these chemical substances. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
TBBPA did not influence osteoblast differentiation, but TCDD suppressed ALP staining and activity, calcium deposition, and osteoblast-related mRNA levels. In a mixture of TBBPA and TCDD, TBBPA inhibited TCDD suppression of adipocyte and osteoblast differentiation in a dose-dependent manner. Interestingly, we observed lipid droplets in TBBPA-treated cells differentiated into osteoblasts. These results suggest that TBBPA and TCDD disrupted differentiation into adipocytes and osteoblasts and contributes to a more complete toxicological understanding of exposure to these chemical substances. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| LPL | Chylomicron-mediated lipid transport | reactome | 17 |
| LPL | D-glyceric acidura | smpdb | 11 |
| LPL | Familial lipoprotein lipase deficiency | smpdb | 11 |
| LPL | FOXA2 and FOXA3 transcription factor networks | pid | 45 |
| LPL | Glycerol Kinase Deficiency | smpdb | 11 |
| LPL | Glycerolipid Metabolism | smpdb | 11 |
| LPL | Retinoid metabolism and transport | reactome | 42 |
| LPL | Transcriptional regulation of white adipocyte differentiation | reactome | 79 |
| LPL | Glycerolipid metabolism | kegg | 49 |
| LPL | PPAR signaling pathway | kegg | 67 |
| LPL | Alzheimer's disease | kegg | 167 |
| LPL | Hs_Angiopoietin_Like_Protein_8_Regulatory_Pathway_WP3915_90629 | wikipathways | 9 |
| LPL | Hs_Composition_of_Lipid_Particles_WP3601_89202 | wikipathways | 5 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene