About Enhancer

Enhancer ID: E_01_0404
Species: human
Position : chr13:40552875-40554875
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Acute promyelocytic leukemia
Pubmed ID:  29911120
Enhancer experiment: Cell transfection,Reverse transcriptase microRNA real-time PCR,qRT-PCR,Cell viability assay,
Enhancer experiment description: Increasing of the apoptotic ratio was linked to miR-182-5p inhibition in the LNA-anti-miR group rather than other groups. Similarly, the necrotic ratio in the LNA-anti-miR group was higher.Our results supported the hypothesis that miR-182-5p inhibition can reduce the cell viability predominantly due to induces apoptosis and necrosis. The present results can apply in translational medicine for investigation of antisense therapy and drug development in leukemia.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: Increasing of the apoptotic ratio was linked to miR-182-5p inhibition in the LNA-anti-miR group rather than other groups. Similarly, the necrotic ratio in the LNA-anti-miR group was higher.Our results supported the hypothesis that miR-182-5p inhibition can reduce the cell viability predominantly due to induces apoptosis and necrosis. The present results can apply in translational medicine for investigation of antisense therapy and drug development in leukemia.

About TF

TF name : FOXO1(FKH1,FKHRA,FOXO1)
TF experiment: Cell transfection,Reverse transcriptase microRNA real-time PCR,qRT-PCR,Cell viability assay,
TF experiment description: Increasing of the apoptotic ratio was linked to miR-182-5p inhibition in the LNA-anti-miR group rather than other groups. Similarly, the necrotic ratio in the LNA-anti-miR group was higher.Our results supported the hypothesis that miR-182-5p inhibition can reduce the cell viability predominantly due to induces apoptosis and necrosis. The present results can apply in translational medicine for investigation of antisense therapy and drug development in leukemia.

About Function

Enhancer function : Increasing of the apoptotic ratio was linked to miR-182-5p inhibition in the LNA-anti-miR group rather than other groups. Similarly, the necrotic ratio in the LNA-anti-miR group was higher.Our results supported the hypothesis that miR-182-5p inhibition can reduce the cell viability predominantly due to induces apoptosis and necrosis. The present results can apply in translational medicine for investigation of antisense therapy and drug development in leukemia.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 Increasing of the apoptotic ratio was linked to miR-182-5p inhibition in the LNA-anti-miR group rather than other groups. Similarly, the necrotic ratio in the LNA-anti-miR group was higher.Our results supported the hypothesis that miR-182-5p inhibition can reduce the cell viability predominantly due to induces apoptosis and necrosis. The present results can apply in translational medicine for investigation of antisense therapy and drug development in leukemia.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
FOXO1 AKT phosphorylates targets in the nucleus reactome 9
FOXO1 AndrogenReceptor netpath 167
FOXO1 Angiopoietin receptor Tie2-mediated signaling pid 50
FOXO1 BCR netpath 161
FOXO1 Class I PI3K signaling events mediated by Akt pid 35
FOXO1 Constitutive Signaling by AKT1 E17K in Cancer reactome 25
FOXO1 CXCR4-mediated signaling events pid 87
FOXO1 EGFR1 netpath 475
FOXO1 FoxO family signaling pid 50
FOXO1 FSH netpath 44
FOXO1 IL-7 netpath 28
FOXO1 IL3 netpath 84
FOXO1 IL6-mediated signaling events pid 48
FOXO1 IL6 netpath 85
FOXO1 Insulin/IGF pathway-protein kinase B signaling cascade panther 22
FOXO1 Leptin netpath 98
FOXO1 MAPK6/MAPK4 signaling reactome 90
FOXO1 Negative regulation of gene expression ( Insulin receptor signaling (D. melanogaster) ) inoh 7
FOXO1 Negative regulation of gene expression ( Insulin receptor signaling (Mammal) ) inoh 7
FOXO1 PI3 kinase pathway panther 38
FOXO1 Regulation of Androgen receptor activity pid 54
FOXO1 Regulation of gene expression in beta cells reactome 17
FOXO1 Regulation of nuclear SMAD2/3 signaling pid 82
FOXO1 Signaling events mediated by HDAC Class III pid 41
FOXO1 TGF_beta_Receptor netpath 220
FOXO1 TNFalpha netpath 274
FOXO1 Insulin signaling pathway kegg 134
FOXO1 Pathways in cancer kegg 321
FOXO1 Prostate cancer kegg 85
FOXO1 Hs_ErbB_Signaling_Pathway_WP673_91869 wikipathways 37
FOXO1 Hs_Angiopoietin_Like_Protein_8_Regulatory_Pathway_WP3915_90629 wikipathways 9
FOXO1 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
FOXO1 Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 wikipathways 153
FOXO1 Hs_Rac1-Pak1-p38-MMP-2_pathway_WP3303_87628 wikipathways 32
FOXO1 Hs_Energy_Metabolism_WP1541_91584 wikipathways 13
FOXO1 Hs_Transcription_factor_regulation_in_adipogenesis_WP3599_88581 wikipathways 9
FOXO1 Hs_NAD_metabolism,_sirtuins_and_aging_WP3630_88178 wikipathways 8
FOXO1 Hs_AGE-RAGE_pathway_WP2324_89798 wikipathways 27
FOXO1 Hs_Follicle_Stimulating_Hormone_(FSH)_signaling_pathway_WP2035_86080 wikipathways 20

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs