About Enhancer

Enhancer ID: E_01_0437
Species: human
Position : chr5:138462604-138464604
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Inflammation, tumour
Pubmed ID:  29858072
Enhancer experiment: Transfection,RT-PCR,Western Blot,GST Pull-Down,Super-EMSA,ChIP,Co-IP,ChIP-3C/ChIP-Loop Assays,Cells Proliferation CCK8 Assay,Soft-Agar Colony Formation Assay,Xenograft Transplantation In Vivo,Histological Analysis
Enhancer experiment description: Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.;Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.;Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.

About TF

TF name : EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)TLR4CTCF(MRD21)
TF experiment: Transfection,RT-PCR,Western Blot,GST Pull-Down,Super-EMSA,ChIP,Co-IP,ChIP-3C/ChIP-Loop Assays,Cells Proliferation CCK8 Assay,Soft-Agar Colony Formation Assay,Xenograft Transplantation In Vivo,Histological Analysis
TF experiment description: Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.;Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.;Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.

About Function

Enhancer function : Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e.g., TNFR1, CLYD, EGR1, NF?B, TLR4, and PPAR?. Furthermore, the P62 cooperates with TNF-? to promote malignant transformation of mesenchymal stem cells. These findings, for the first time, provide insight into the positive role that P62 plays in malignant transformation of mesenchymal stem cells and reveal a novel link between P62 and the inflammation factors in mesenchymal stem cells.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
EGR1 AP-1 transcription factor network pid 71
EGR1 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
EGR1 Downstream signaling in nave CD8+ T cells pid 69
EGR1 ErbB1 downstream signaling pid 105
EGR1 Glucocorticoid receptor regulatory network pid 85
EGR1 Interferon alpha/beta signaling reactome 67
EGR1 Regulation of Androgen receptor activity pid 54
EGR1 Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met) pid 80
EGR1 Signaling events mediated by PRL pid 23
EGR1 TCR netpath 261
EGR1 Trk receptor signaling mediated by PI3K and PLC-gamma pid 36
EGR1 Trk receptor signaling mediated by the MAPK pathway pid 34
EGR1 Prion diseases kegg 34
EGR1 Hs_Thyroid_Stimulating_Hormone_(TSH)_signaling_pathway_WP2032_89823 wikipathways 28
EGR1 Hs_Serotonin_Receptor_4-6-7_and_NR3C_Signaling_WP734_74438 wikipathways 15
EGR1 Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
TLR4 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon reactome 18
TLR4 Endogenous TLR signaling pid 26
TLR4 IKK complex recruitment mediated by RIP1 reactome 24
TLR4 IL-1_signaling(through_IKK-NFkB_cascade)(canonical) ( IL-1 signaling pathway (through NF-kappaB) ) inoh 15
TLR4 IRAK4 deficiency (TLR2/4) reactome 11
TLR4 Ligand-dependent caspase activation reactome 16
TLR4 MyD88-independent TLR3/TLR4 cascade reactome 7
TLR4 MyD88 deficiency (TLR2/4) reactome 11
TLR4 MyD88:Mal cascade initiated on plasma membrane reactome 19
TLR4 Toll-like receptor signaling pathway (p38 cascade) ( Toll-like receptor signaling pathway (p38 cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, JNK cascade) ( Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, JNK cascade) ) inoh 15
TLR4 Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, p38 cascade) ( Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, p38 cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through JNK cascade)(Canonical) ( Toll-like receptor signaling pathway (through JNK cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through LPS, TLR4, MyD88, IRAK, TAK1 and IKK-NF-kappaB cascade)(Canonical) ( Toll-like receptor signaling pathway (trough NF-kappaB) ) inoh 19
TLR4 Toll Like Receptor 4 (TLR4) Cascade reactome 15
TLR4 Toll receptor signaling pathway panther 44
TLR4 TRAF6 mediated induction of TAK1 complex reactome 16
TLR4 TRIF-mediated programmed cell death reactome 10
TLR4 Phagosome kegg 154
TLR4 Toll-like receptor signaling pathway kegg 100
TLR4 Pathogenic Escherichia coli infection kegg 49
TLR4 Leishmaniasis kegg 71
TLR4 Chagas disease kegg 103
TLR4 Malaria kegg 50
TLR4 Toxoplasmosis kegg 131
TLR4 Amoebiasis kegg 103
TLR4 Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 wikipathways 41
TLR4 Hs_Regulation_of_toll-like_receptor_signaling_pathway_WP1449_81172 wikipathways 47
TLR4 Hs_Pathogenic_Escherichia_coli_infection_WP2272_78594 wikipathways 7
TLR4 Hs_Toll-like_Receptor_Signaling_WP3858_89945 wikipathways 7
CTCF Activation of anterior HOX genes in hindbrain development during early embryogenesis reactome 120
CTCF TGF_beta_Receptor netpath 220

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs