About Enhancer

Enhancer ID: E_01_0445
Species: human
Position : chr17:34252611-34254611
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Prostate cancer (pca)
Pubmed ID:  29808619
Enhancer experiment: ChiP-seq,ChIP-seq,western blot,immunohistochemistry (IHC)
Enhancer experiment description: AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.;AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.;AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.

About TF

TF name : CCL2CXCL8AR(AIS8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM,AR)
TF experiment: ChiP-seq,ChIP-seq,western blot,immunohistochemistry (IHC)
TF experiment description: AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.;AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.;AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.

About Function

Enhancer function : AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 AR signaling in CAF-like cells decreased expression of multiple cytokines; most notably CCL2 and CXCL8 and both cytokines increased migration of PCa cells. These results suggest direct paracrine regulation of PCa cell migration by CAFs through AR signaling.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
CCL2 AP-1 transcription factor network pid 71
CCL2 ATF4 activates genes reactome 25
CCL2 Chemokine receptors bind chemokines reactome 57
CCL2 GMCSF-mediated signaling events pid 38
CCL2 IL23-mediated signaling events pid 37
CCL2 Inflammation mediated by chemokine and cytokine signaling pathway panther 189
CCL2 Validated transcriptional targets of AP1 family members Fra1 and Fra2 pid 37
CCL2 Chemokine signaling pathway kegg 187
CCL2 NOD-like receptor signaling pathway kegg 60
CCL2 Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
CCL2 Hs_Folate_Metabolism_WP176_85063 wikipathways 33
CCL2 Hs_Vitamin_B12_Metabolism_WP1533_85340 wikipathways 33
CCL2 Hs_Lung_fibrosis_WP3624_91475 wikipathways 23
CCL2 Hs_TNF_alpha_Signaling_Pathway_WP231_89895 wikipathways 61
CCL2 Hs_Selenium_Micronutrient_Network_WP15_88908 wikipathways 35
CCL2 Hs_IL-1_signaling_pathway_WP195_89932 wikipathways 34
CXCL8 AP-1 transcription factor network pid 71
CXCL8 ATF-2 transcription factor network pid 59
CXCL8 ATF4 activates genes reactome 25
CXCL8 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
CXCL8 Chemokine receptors bind chemokines reactome 57
CXCL8 G alpha (i) signalling events reactome 241
CXCL8 Glucocorticoid receptor regulatory network pid 85
CXCL8 Heterotrimeric GPCR signaling pathway (through G alpha i and pertussis toxin) ( GPCR signaling (pertussis toxin) ) inoh 228
CXCL8 Heterotrimeric GPCR signaling pathway (through G alpha q, PLC beta and ERK cascade) ( GPCR signaling (G alpha q) ) inoh 239
CXCL8 Heterotrimeric GPCR signaling pathway (through G alpha s ACs Epac BRaf and ERKcascade) ( GPCR signaling (G alpha s, Epac and ERK) ) inoh 237
CXCL8 Heterotrimeric GPCR signaling pathway (through_G alpha s_ACs_PKA_BRaf_and_ERKcascade)(canonical) ( GPCR signaling (G alpha s, PKA and ERK) ) inoh 227
CXCL8 Heterotrimeric GTP-binding protein coupled receptor signaling pathway (through G alpha i, adenylate cyclase and cAMP) ( GPCR signaling (G alpha i) ) inoh 228
CXCL8 Heterotrimeric GTP-binding protein coupled receptor signaling pathway (through_G_alpha_s,_cholera_toxin,_adenylate_cyclase_and_cAMP) ( GPCR signaling (cholera toxin) ) inoh 227
CXCL8 IL8- and CXCR1-mediated signaling events pid 29
CXCL8 IL8- and CXCR2-mediated signaling events pid 35
CXCL8 Inflammation mediated by chemokine and cytokine signaling pathway panther 189
CXCL8 Interleukin signaling pathway panther 86
CXCL8 JAK-STAT pathway and regulation pathway ( JAK-STAT pathway and regulation pathway Diagram ) inoh 97
CXCL8 LPA receptor mediated events pid 65
CXCL8 Peptide ligand-binding receptors reactome 103
CXCL8 Regulation of nuclear beta catenin signaling and target gene transcription pid 80
CXCL8 Senescence-Associated Secretory Phenotype (SASP) reactome 108
CXCL8 Syndecan-2-mediated signaling events pid 38
CXCL8 Syndecan-3-mediated signaling events pid 23
CXCL8 Validated transcriptional targets of AP1 family members Fra1 and Fra2 pid 37
CXCL8 Hs_Hepatitis_C_and_Hepatocellular_Carcinoma_WP3646_88640 wikipathways 36
CXCL8 Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193 wikipathways 62
AR Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 reactome 65
AR Alpha6Beta4Integrin netpath 74
AR AndrogenReceptor netpath 167
AR Coregulation of Androgen receptor activity pid 61
AR FOXA1 transcription factor network pid 45
AR IL6 netpath 85
AR Nongenotropic Androgen signaling pid 31
AR Notch-mediated HES/HEY network pid 48
AR Nuclear Receptor transcription pathway reactome 51
AR Regulation of Androgen receptor activity pid 54
AR Regulation of nuclear beta catenin signaling and target gene transcription pid 80
AR Regulation of nuclear SMAD2/3 signaling pid 82
AR TGF_beta_Receptor netpath 220
AR Oocyte meiosis kegg 110
AR Pathways in cancer kegg 321
AR Prostate cancer kegg 85
AR Hs_DNA_Damage_Response_WP707_82937 wikipathways 28
AR Hs_miRNA_Regulation_of_DNA_Damage_Response_WP1530_84694 wikipathways 28
AR Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
AR Hs_Sudden_Infant_Death_Syndrome_(SIDS)_Susceptibility_Pathways_WP706_86078 wikipathways 33
AR Hs_Androgen_receptor_signaling_pathway_WP138_79958 wikipathways 27

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs