About Enhancer

Enhancer ID: E_01_0446
Species: human
Position : chr4:106918996-106920996
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Facet joint osteoarthritis, lumbar spine osteoarthritis
Pubmed ID:  29806631
Enhancer experiment: RNA-seq,Bioinformatic analysis,qRT-PCR,
Enhancer experiment description: In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.

About Target gene

Target gene : DKK2,TCF7,CCL4,CCL4L2
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.

About TF

TF name : SOX17(Sox17)MYC(MRTLC,bHLHe39,c-Myc,MYC)CAMK2ALEF1(LEF-1,TCF10,TCF1ALPHA,TCF7L3)
TF experiment: RNA-seq,Bioinformatic analysis,qRT-PCR,
TF experiment description: In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.;In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.

About Function

Enhancer function : In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 In NF-?B signaling pathway, the mRNA levels of C-C Motif Chemokine Ligand 4 (CCL4) and C-C Motif Chemokine Ligand 4 Like 2 (CCL4L2) were increased, while the mRNA levels of BCL2 Related Protein A1 were decreased. These results suggest that Wnt and NF-?B signaling may be altered in the process of facet joint cartilage degeneration. The present study will expand our understanding of the molecular bases underlying facet joint osteoarthritis.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
SOX17 Deactivation of the beta-catenin transactivating complex reactome 42
SOX17 Wnt signaling pathway kegg 135
SOX17 Hs_Endoderm_Differentiation_WP2853_88152 wikipathways 62
MYC AP-1 transcription factor network pid 71
MYC Binding of TCF/LEF:CTNNB1 to target gene promoters reactome 7
MYC C-MYB transcription factor network pid 87
MYC C-MYC pathway pid 25
MYC CD40/CD40L signaling pid 36
MYC Ceramide signaling pathway pid 49
MYC Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants reactome 57
MYC Constitutive Signaling by NOTCH1 PEST Domain Mutants reactome 57
MYC Cyclin A:Cdk2-associated events at S phase entry reactome 15
MYC Cyclin E associated events during G1/S transition reactome 14
MYC E2F transcription factor network pid 77
MYC EGFR1 netpath 475
MYC Formation of the beta-catenin:TCF transactivating complex reactome 88
MYC FOXM1 transcription factor network pid 43
MYC IL2-mediated signaling events pid 54
MYC IL2 signaling events mediated by PI3K pid 38
MYC IL2 signaling events mediated by STAT5 pid 30
MYC IL6-mediated signaling events pid 48
MYC Interleukin signaling pathway panther 86
MYC LKB1 signaling events pid 43
MYC MAPK6/MAPK4 signaling reactome 90
MYC Notch signaling pathway pid 58
MYC NOTCH1 Intracellular Domain Regulates Transcription reactome 47
MYC Oxidative stress response panther 23
MYC p53 pathway feedback loops 2 panther 42
MYC p73 transcription factor network pid 80
MYC PDGF signaling pathway panther 113
MYC PDGFR-beta signaling pathway pid 125
MYC Presenilin action in Notch and Wnt signaling pid 46
MYC Prolactin netpath 105
MYC Regulation of nuclear beta catenin signaling and target gene transcription pid 80
MYC Regulation of nuclear SMAD2/3 signaling pid 82
MYC Regulation of Telomerase pid 70
MYC SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription reactome 32
MYC TGF_beta_Receptor netpath 220
MYC Validated nuclear estrogen receptor alpha network pid 65
MYC Validated targets of C-MYC transcriptional activation pid 80
MYC Validated targets of C-MYC transcriptional repression pid 63
MYC MAPK signaling pathway kegg 264
MYC ErbB signaling pathway kegg 83
MYC Cell cycle kegg 124
MYC Wnt signaling pathway kegg 135
MYC TGF-beta signaling pathway kegg 82
MYC Jak-STAT signaling pathway kegg 149
MYC Pathways in cancer kegg 321
MYC Colorectal cancer kegg 63
MYC Endometrial cancer kegg 52
MYC Thyroid cancer kegg 25
MYC Bladder cancer kegg 38
MYC Chronic myeloid leukemia kegg 69
MYC Acute myeloid leukemia kegg 53
MYC Small cell lung cancer kegg 83
MYC Hs_DNA_Damage_Response_(only_ATM_dependent)_WP710_79974 wikipathways 36
MYC Hs_TP53_Network_WP1742_71700 wikipathways 10
MYC Hs_Imatinib_and_Chronic_Myeloid_Leukemia_WP3640_89384 wikipathways 11
MYC Hs_miRNAs_involved_in_DNA_damage_response_WP1545_84697 wikipathways 30
MYC Hs_Association_Between_Physico-Chemical_Features_and_Toxicity_Associated_Pathways_WP3680_90113 wikipathways 31
MYC Hs_Gastric_Cancer_Network_2_WP2363_87523 wikipathways 28
MYC Hs_Hepatitis_C_and_Hepatocellular_Carcinoma_WP3646_88640 wikipathways 36
MYC Hs_Apoptosis_WP254_88977 wikipathways 31
MYC Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
MYC Hs_Neural_Crest_Differentiation_WP2064_79263 wikipathways 40
MYC Hs_IL-5_Signaling_Pathway_WP127_78498 wikipathways 35
MYC Hs_Prolactin_Signaling_Pathway_WP2037_90015 wikipathways 52
MYC Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 wikipathways 107
MYC Hs_IL-7_Signaling_Pathway_WP205_89854 wikipathways 16
CAMK2A AKT(PKB)-Bad signaling ( EPO signaling pathway(JAK2 STAT1 STAT3 STAT5) ) inoh 173
CAMK2A AKT(PKB)-Bad signaling ( IL-7 signaling(JAK1 JAK3 STAT5) ) inoh 173
CAMK2A Ca2+ pathway reactome 62
CAMK2A CREB phosphorylation through the activation of CaMKII reactome 17
CAMK2A CREB phosphorylation through the activation of Ras reactome 13
CAMK2A Drosophila Toll-like receptor signaling ( Drosophila Toll-like receptor signaling ) inoh 236
CAMK2A EGFR1 netpath 475
CAMK2A FGF signaling pathway pid 55
CAMK2A HSF1-dependent transactivation reactome 34
CAMK2A IFN-gamma pathway pid 43
CAMK2A Inflammation mediated by chemokine and cytokine signaling pathway panther 189
CAMK2A Interferon gamma signaling reactome 88
CAMK2A Ion homeostasis reactome 53
CAMK2A Ion transport by P-type ATPases reactome 57
CAMK2A Ionotropic glutamate receptor pathway panther 42
CAMK2A Netrin-mediated signaling events pid 32
CAMK2A Noncanonical Wnt signaling pathway pid 33
CAMK2A Phase 0 - rapid depolarisation reactome 46
CAMK2A Positive regulation of (Transcription of SOCS by STAT dimer) in JAK STAT pathway ( JAK-STAT pathway and regulation pathway Diagram ) inoh 178
CAMK2A RAF/MAP kinase cascade reactome 116
CAMK2A Ras activation uopn Ca2+ infux through NMDA receptor reactome 19
CAMK2A Signaling events mediated by HDAC Class II pid 58
CAMK2A TGF-beta receptor signaling pid 53
CAMK2A TGF_beta_Receptor netpath 220
CAMK2A Trafficking of AMPA receptors reactome 17
CAMK2A Trk receptor signaling mediated by PI3K and PLC-gamma pid 36
CAMK2A Unblocking of NMDA receptor, glutamate binding and activation reactome 17
CAMK2A VEGF signaling pathway ( VEGF signaling pathway ) inoh 183
CAMK2A ErbB signaling pathway kegg 83
CAMK2A Calcium signaling pathway kegg 173
CAMK2A Oocyte meiosis kegg 110
CAMK2A Wnt signaling pathway kegg 135
CAMK2A Long-term potentiation kegg 67
CAMK2A Neurotrophin signaling pathway kegg 126
CAMK2A Olfactory transduction kegg 381
CAMK2A Phototransduction - fly kegg 26
CAMK2A GnRH signaling pathway kegg 98
CAMK2A Melanogenesis kegg 100
CAMK2A Glioma kegg 65
CAMK2A Hs_Common_Pathways_Underlying_Drug_Addiction_WP2636_89423 wikipathways 9
CAMK2A Hs_Melatonin_metabolism_and_effects_WP3298_91618 wikipathways 7
CAMK2A Hs_Nucleotide_Metabolism_WP404_68960 wikipathways 16
CAMK2A Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900 wikipathways 71
LEF1 Alzheimer disease-presenilin pathway panther 98
LEF1 Binding of TCF/LEF:CTNNB1 to target gene promoters reactome 7
LEF1 C-MYB transcription factor network pid 87
LEF1 Ca2+ pathway reactome 62
LEF1 Cadherin signaling pathway panther 147
LEF1 Canonical Wnt signaling pathway ( C. elegans endoderm induction Wnt signaling pathway Diagram ) inoh 56
LEF1 Canonical Wnt signaling pathway ( Canonical Wnt signaling pathway Diagram ) inoh 56
LEF1 Canonical Wnt signaling pathway ( Drosophila Wingless/Wnt signaling pathway Diagram ) inoh 56
LEF1 Canonical Wnt signaling pathway ( Mammalian Wnt signaling pathway Diagram ) inoh 56
LEF1 Canonical Wnt signaling pathway ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 56
LEF1 Deactivation of the beta-catenin transactivating complex reactome 42
LEF1 Formation of the beta-catenin:TCF transactivating complex reactome 88
LEF1 Integrin-linked kinase signaling pid 46
LEF1 Mammalian Wnt signaling pathway ( Mammalian Wnt signaling pathway Diagram ) inoh 49
LEF1 Notch netpath 76
LEF1 Regulation of nuclear beta catenin signaling and target gene transcription pid 80
LEF1 Repression of WNT target genes reactome 14
LEF1 Signaling with Wnt (Canonical) ( C. elegans endoderm induction Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Canonical) ( Canonical Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Canonical) ( Drosophila Wingless/Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Canonical) ( Mammalian Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Canonical) ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Mammal) ( Mammalian Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Xenopus) ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 19
LEF1 Signaling without Wnt (Canonical) ( C. elegans endoderm induction Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Canonical) ( Canonical Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Canonical) ( Drosophila Wingless/Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Canonical) ( Mammalian Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Canonical) ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Mammal) ( Mammalian Wnt signaling pathway Diagram ) inoh 8
LEF1 TGF_beta_Receptor netpath 220
LEF1 Wnt netpath 118
LEF1 Wnt signaling pathway panther 250
LEF1 Wnt signaling pathway kegg 135
LEF1 Adherens junction kegg 70
LEF1 Melanogenesis kegg 100
LEF1 Pathways in cancer kegg 321
LEF1 Colorectal cancer kegg 63
LEF1 Endometrial cancer kegg 52
LEF1 Prostate cancer kegg 85
LEF1 Acute myeloid leukemia kegg 53
LEF1 Arrhythmogenic right ventricular cardiomyopathy (ARVC) kegg 71
LEF1 Hs_Wnt_Signaling_Pathway_Netpath_WP363_78571 wikipathways 28
LEF1 Hs_Endoderm_Differentiation_WP2853_88152 wikipathways 62
LEF1 Hs_Mesodermal_Commitment_Pathway_WP2857_87780 wikipathways 47

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs