About Enhancer

Enhancer ID: E_01_0458
Species: human
Position : chr14:75276080-75278080
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Nothing
Pubmed ID:  29789579
Enhancer experiment: CRISPR\Cas9,PCR,western blot,qRT-PCR,immunoprecipitation(IP),Immunostaining,immunoblotting,ChIP assays,ChIP-PCR,MS analysis,RNA-Seq,western blotting (WB) analysis
Enhancer experiment description: Taken together, we resolve the long-standing controversy and uncertainty in the field, and reveal a fundamental role of OCT4A protein in regulating FOS/AP-1 signaling-centered genes that mediate the adhesion, migration, and propagation of somatic cancer cells.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: Taken together, we resolve the long-standing controversy and uncertainty in the field, and reveal a fundamental role of OCT4A protein in regulating FOS/AP-1 signaling-centered genes that mediate the adhesion, migration, and propagation of somatic cancer cells.;Taken together, we resolve the long-standing controversy and uncertainty in the field, and reveal a fundamental role of OCT4A protein in regulating FOS/AP-1 signaling-centered genes that mediate the adhesion, migration, and propagation of somatic cancer cells.

About TF

TF name : FOS(AP-1,C-FOS, p55)POU5F1(OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4)
TF experiment: CRISPR\Cas9,PCR,western blot,qRT-PCR,immunoprecipitation(IP),Immunostaining,immunoblotting,ChIP assays,ChIP-PCR,MS analysis,RNA-Seq,western blotting (WB) analysis
TF experiment description: Taken together, we resolve the long-standing controversy and uncertainty in the field, and reveal a fundamental role of OCT4A protein in regulating FOS/AP-1 signaling-centered genes that mediate the adhesion, migration, and propagation of somatic cancer cells.;Taken together, we resolve the long-standing controversy and uncertainty in the field, and reveal a fundamental role of OCT4A protein in regulating FOS/AP-1 signaling-centered genes that mediate the adhesion, migration, and propagation of somatic cancer cells.

About Function

Enhancer function : Taken together, we resolve the long-standing controversy and uncertainty in the field, and reveal a fundamental role of OCT4A protein in regulating FOS/AP-1 signaling-centered genes that mediate the adhesion, migration, and propagation of somatic cancer cells.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 Taken together, we resolve the long-standing controversy and uncertainty in the field, and reveal a fundamental role of OCT4A protein in regulating FOS/AP-1 signaling-centered genes that mediate the adhesion, migration, and propagation of somatic cancer cells.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
FOS Activation of the AP-1 family of transcription factors reactome 10
FOS Alpha6Beta4Integrin netpath 74
FOS Angiogenesis panther 141
FOS AP-1 transcription factor network pid 71
FOS Apoptosis signaling pathway panther 104
FOS ATF-2 transcription factor network pid 59
FOS B cell activation panther 52
FOS BCR netpath 161
FOS BCR signaling pathway pid 70
FOS Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
FOS Calcium signaling in the CD4+ TCR pathway pid 29
FOS Downstream signaling in nave CD8+ T cells pid 69
FOS EGFR1 netpath 475
FOS Endothelins pid 64
FOS ErbB1 downstream signaling pid 105
FOS ErbB2/ErbB3 signaling events pid 41
FOS Fc-epsilon receptor I signaling in mast cells pid 64
FOS FCERI mediated MAPK activation reactome 40
FOS FGF signaling pathway pid 55
FOS FOXA1 transcription factor network pid 45
FOS FOXM1 transcription factor network pid 43
FOS Gene expression of IL2 by AP-1 ( CD4 T cell receptor signaling (JNK cascade) ) inoh 5
FOS Gene expression of IL2 by AP-1 ( CD4 T cell receptor signaling ) inoh 5
FOS Glucocorticoid receptor regulatory network pid 85
FOS GMCSF-mediated signaling events pid 38
FOS HIF-1-alpha transcription factor network pid 67
FOS Huntington disease panther 113
FOS IL12-mediated signaling events pid 62
FOS IL12 signaling mediated by STAT4 pid 33
FOS IL2-mediated signaling events pid 54
FOS IL6-mediated signaling events pid 48
FOS IL6 netpath 85
FOS Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade panther 29
FOS Interleukin signaling pathway panther 86
FOS JNK cascade ( CD4 T cell receptor signaling (JNK cascade) ) inoh 12
FOS JNK cascade ( CD4 T cell receptor signaling ) inoh 10
FOS LPA receptor mediated events pid 65
FOS Nongenotropic Androgen signaling pid 31
FOS Osteopontin-mediated events pid 32
FOS Oxidative Stress Induced Senescence reactome 120
FOS PDGF signaling pathway panther 113
FOS PDGFR-alpha signaling pathway pid 22
FOS PDGFR-beta signaling pathway pid 125
FOS Presenilin action in Notch and Wnt signaling pid 46
FOS Prolactin netpath 105
FOS Ras signaling in the CD4+ TCR pathway pid 14
FOS Regulation of nuclear SMAD2/3 signaling pid 82
FOS Regulation of Telomerase pid 70
FOS RhoA signaling pathway pid 46
FOS S1P2 pathway pid 26
FOS Senescence-Associated Secretory Phenotype (SASP) reactome 108
FOS T cell activation panther 60
FOS TCR netpath 261
FOS TGF_beta_Receptor netpath 220
FOS TP53 Regulates Transcription of DNA Repair Genes reactome 61
FOS Trk receptor signaling mediated by the MAPK pathway pid 34
FOS MAPK signaling pathway kegg 264
FOS Toll-like receptor signaling pathway kegg 100
FOS T cell receptor signaling pathway kegg 105
FOS B cell receptor signaling pathway kegg 70
FOS Leishmaniasis kegg 71
FOS Chagas disease kegg 103
FOS Pathways in cancer kegg 321
FOS Colorectal cancer kegg 63
FOS Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 wikipathways 41
FOS Hs_Apoptosis_Modulation_and_Signaling_WP1772_91293 wikipathways 71
FOS Hs_Serotonin_and_anxiety-related_events_WP3944_90836 wikipathways 9
FOS Hs_Structural_Pathway_of_Interleukin_1_(IL-1)_WP2637_87707 wikipathways 34
FOS Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
FOS Hs_Estrogen_signaling_pathway_WP712_78491 wikipathways 13
FOS Hs_IL-5_Signaling_Pathway_WP127_78498 wikipathways 35
FOS Hs_IL-4_Signaling_Pathway_WP395_89828 wikipathways 41
FOS Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 wikipathways 107
FOS Hs_IL-3_Signaling_Pathway_WP286_78583 wikipathways 35
POU5F1 HIF-2-alpha transcription factor network pid 34
POU5F1 POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation reactome 13
POU5F1 POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation reactome 10
POU5F1 Transcriptional regulation of pluripotent stem cells reactome 28
POU5F1 Hs_Endoderm_Differentiation_WP2853_88152 wikipathways 62
POU5F1 Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 wikipathways 12

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs