About Enhancer

Enhancer ID: E_01_0460
Species: human
Position : chr6:170551706-170553706
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Nothing
Pubmed ID:  29785964
Enhancer experiment: transfection,immunoblots,DNase I footprinting,EMSAs,In vitro transcription assay,ChIPs,Pull-down assay,ChIP-seq,ChIP-qPCRs
Enhancer experiment description: In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.

About Target gene

Target gene : BRF2
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.;In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.

About TF

TF name : TBP(GTF2D1,Gtf2d,SCA17,TFIID)
TF experiment: transfection,immunoblots,DNase I footprinting,EMSAs,In vitro transcription assay,ChIPs,Pull-down assay,ChIP-seq,ChIP-qPCRs
TF experiment description: In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.;In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.

About Function

Enhancer function : In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
TBP B-WICH complex positively regulates rRNA expression reactome 89
TBP General transcription by RNA polymerase I panther 15
TBP Glucocorticoid receptor regulatory network pid 85
TBP HIV Transcription Initiation reactome 44
TBP Huntington disease panther 113
TBP NoRC negatively regulates rRNA expression reactome 104
TBP Regulation of retinoblastoma protein pid 67
TBP Regulation of TP53 Activity through Phosphorylation reactome 89
TBP RNA Polymerase I Chain Elongation reactome 88
TBP RNA Polymerase I Promoter Escape reactome 30
TBP RNA Polymerase I Transcription Initiation reactome 47
TBP RNA Polymerase I Transcription Termination reactome 31
TBP RNA Polymerase II HIV Promoter Escape reactome 44
TBP RNA Polymerase II Pre-transcription Events reactome 64
TBP RNA Polymerase II Promoter Escape reactome 44
TBP RNA polymerase II transcribes snRNA genes reactome 70
TBP RNA Polymerase II Transcription Initiation And Promoter Clearance reactome 44
TBP RNA Polymerase II Transcription Initiation reactome 44
TBP RNA Polymerase II Transcription Pre-Initiation And Promoter Opening reactome 44
TBP RNA Polymerase III Abortive And Retractive Initiation reactome 41
TBP RNA Polymerase III Transcription Initiation From Type 1 Promoter reactome 28
TBP RNA Polymerase III Transcription Initiation From Type 2 Promoter reactome 27
TBP RNA Polymerase III Transcription Initiation From Type 3 Promoter reactome 28
TBP SIRT1 negatively regulates rRNA Expression reactome 66
TBP Transcription of the HIV genome reactome 44
TBP Transcription regulation by bZIP transcription factor panther 42
TBP Validated targets of C-MYC transcriptional repression pid 63
TBP Huntington's disease kegg 182

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs