About Enhancer
| Enhancer ID: | E_01_0474 |
| Species: | human |
| Position : | chr7:148804347-148806347   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Nothing |
| Pubmed ID: | 29768655 |
| Enhancer experiment: | Western blot,Immunofluorescence assay,qRT-PCR,ELISA,Luciferase reporter assay,iTRAQ,miRNA or siRNA transfection,EZH2 knockdown,Smad3 knockdown |
| Enhancer experiment description: | More importantly, we revealed that siEZH2 down-regulated NF-?B p65 within the nucleus. These findings indicate that the let-7e/EZH2/H3K27me3/NF-?B p65 pathway is a novel regulatory axis of TNF? expression. In addition, we determined the protein differences between siEZH2 and siEZH2-NC by iTRAQ and found a number of proteins that might be associated with TNF?. |
About Target gene
| Target gene : | Smad3 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | More importantly, we revealed that siEZH2 down-regulated NF-?B p65 within the nucleus. These findings indicate that the let-7e/EZH2/H3K27me3/NF-?B p65 pathway is a novel regulatory axis of TNF? expression. In addition, we determined the protein differences between siEZH2 and siEZH2-NC by iTRAQ and found a number of proteins that might be associated with TNF?.;More importantly, we revealed that siEZH2 down-regulated NF-?B p65 within the nucleus. These findings indicate that the let-7e/EZH2/H3K27me3/NF-?B p65 pathway is a novel regulatory axis of TNF? expression. In addition, we determined the protein differences between siEZH2 and siEZH2-NC by iTRAQ and found a number of proteins that might be associated with TNF?. |
About TF
| TF name : | EZH2(ENX-1,ENX1b,KMT6,KMT6A,WVS,WVS2,EZH2) |
| TF experiment: | Western blot,Immunofluorescence assay,qRT-PCR,ELISA,Luciferase reporter assay,iTRAQ,miRNA or siRNA transfection,EZH2 knockdown,Smad3 knockdown |
| TF experiment description: | More importantly, we revealed that siEZH2 down-regulated NF-?B p65 within the nucleus. These findings indicate that the let-7e/EZH2/H3K27me3/NF-?B p65 pathway is a novel regulatory axis of TNF? expression. In addition, we determined the protein differences between siEZH2 and siEZH2-NC by iTRAQ and found a number of proteins that might be associated with TNF?.;More importantly, we revealed that siEZH2 down-regulated NF-?B p65 within the nucleus. These findings indicate that the let-7e/EZH2/H3K27me3/NF-?B p65 pathway is a novel regulatory axis of TNF? expression. In addition, we determined the protein differences between siEZH2 and siEZH2-NC by iTRAQ and found a number of proteins that might be associated with TNF?. |
About Function
| Enhancer function : | More importantly, we revealed that siEZH2 down-regulated NF-?B p65 within the nucleus. These findings indicate that the let-7e/EZH2/H3K27me3/NF-?B p65 pathway is a novel regulatory axis of TNF? expression. In addition, we determined the protein differences between siEZH2 and siEZH2-NC by iTRAQ and found a number of proteins that might be associated with TNF?. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
More importantly, we revealed that siEZH2 down-regulated NF-?B p65 within the nucleus. These findings indicate that the let-7e/EZH2/H3K27me3/NF-?B p65 pathway is a novel regulatory axis of TNF? expression. In addition, we determined the protein differences between siEZH2 and siEZH2-NC by iTRAQ and found a number of proteins that might be associated with TNF?. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| EZH2 | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
| EZH2 | Oxidative Stress Induced Senescence | reactome | 120 |
| EZH2 | PKMTs methylate histone lysines | reactome | 64 |
| EZH2 | PRC2 methylates histones and DNA | reactome | 71 |
| EZH2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| EZH2 | Hs_Interactome_of_polycomb_repressive_complex_2_(PRC2)_WP2916_88672 | wikipathways | 15 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene