About Enhancer

Enhancer ID: E_01_0479
Species: human
Position : chr9:117701608-117703608
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Inflammation in huvecs
Pubmed ID:  29762844
Enhancer experiment: Flow Cytometry,ELISA,qPCR,Western Blot,Cell Apoptosis Assay,Viability Assay
Enhancer experiment description: TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.;TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.;TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.

About TF

TF name : TLR4CCL5CXCL10
TF experiment: Flow Cytometry,ELISA,qPCR,Western Blot,Cell Apoptosis Assay,Viability Assay
TF experiment description: TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.;TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.;TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.

About Function

Enhancer function : TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 TLR4 mediates FFAs induced inflammatory responses in HUVECs. TLR4 interference in HUVECs significantly reduces the inflammatory cytokines expression, decreases the cell apoptosis rate and increases cell viability.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
TLR4 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon reactome 18
TLR4 Endogenous TLR signaling pid 26
TLR4 IKK complex recruitment mediated by RIP1 reactome 24
TLR4 IL-1_signaling(through_IKK-NFkB_cascade)(canonical) ( IL-1 signaling pathway (through NF-kappaB) ) inoh 15
TLR4 IRAK4 deficiency (TLR2/4) reactome 11
TLR4 Ligand-dependent caspase activation reactome 16
TLR4 MyD88-independent TLR3/TLR4 cascade reactome 7
TLR4 MyD88 deficiency (TLR2/4) reactome 11
TLR4 MyD88:Mal cascade initiated on plasma membrane reactome 19
TLR4 Toll-like receptor signaling pathway (p38 cascade) ( Toll-like receptor signaling pathway (p38 cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, JNK cascade) ( Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, JNK cascade) ) inoh 15
TLR4 Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, p38 cascade) ( Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, p38 cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through JNK cascade)(Canonical) ( Toll-like receptor signaling pathway (through JNK cascade) ) inoh 14
TLR4 Toll-like receptor signaling pathway (through LPS, TLR4, MyD88, IRAK, TAK1 and IKK-NF-kappaB cascade)(Canonical) ( Toll-like receptor signaling pathway (trough NF-kappaB) ) inoh 19
TLR4 Toll Like Receptor 4 (TLR4) Cascade reactome 15
TLR4 Toll receptor signaling pathway panther 44
TLR4 TRAF6 mediated induction of TAK1 complex reactome 16
TLR4 TRIF-mediated programmed cell death reactome 10
TLR4 Phagosome kegg 154
TLR4 Toll-like receptor signaling pathway kegg 100
TLR4 Pathogenic Escherichia coli infection kegg 49
TLR4 Leishmaniasis kegg 71
TLR4 Chagas disease kegg 103
TLR4 Malaria kegg 50
TLR4 Toxoplasmosis kegg 131
TLR4 Amoebiasis kegg 103
TLR4 Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 wikipathways 41
TLR4 Hs_Regulation_of_toll-like_receptor_signaling_pathway_WP1449_81172 wikipathways 47
TLR4 Hs_Pathogenic_Escherichia_coli_infection_WP2272_78594 wikipathways 7
TLR4 Hs_Toll-like_Receptor_Signaling_WP3858_89945 wikipathways 7
CCL5 Chemokine receptors bind chemokines reactome 57
CCL5 G alpha (i) signalling events reactome 241
CCL5 Inflammation mediated by chemokine and cytokine signaling pathway panther 189
CCL5 Syndecan-1-mediated signaling events pid 42
CCL5 Syndecan-4-mediated signaling events pid 32
CCL5 Validated targets of C-MYC transcriptional repression pid 63
CCL5 Cytokine-cytokine receptor interaction kegg 213
CCL5 Chemokine signaling pathway kegg 187
CCL5 Toll-like receptor signaling pathway kegg 100
CCL5 NOD-like receptor signaling pathway kegg 60
CCL5 Cytosolic DNA-sensing pathway kegg 56
CCL5 Prion diseases kegg 34
CCL5 Epithelial cell signaling in Helicobacter pylori infection kegg 67
CCL5 Chagas disease kegg 103
CCL5 Hs_T-Cell_antigen_Receptor_(TCR)_pathway_during_Staphylococcus_aureus_infection_WP3863_90702 wikipathways 35
CCL5 Hs_Vitamin_B12_Metabolism_WP1533_85340 wikipathways 33
CCL5 Hs_Lung_fibrosis_WP3624_91475 wikipathways 23
CXCL10 Chemokine receptors bind chemokines reactome 57
CXCL10 CXCR3-mediated signaling events pid 38
CXCL10 G alpha (i) signalling events reactome 241
CXCL10 Inflammation mediated by chemokine and cytokine signaling pathway panther 189
CXCL10 Cytokine-cytokine receptor interaction kegg 213
CXCL10 Chemokine signaling pathway kegg 187
CXCL10 Toll-like receptor signaling pathway kegg 100
CXCL10 RIG-I-like receptor signaling pathway kegg 71
CXCL10 Cytosolic DNA-sensing pathway kegg 56

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs