About Enhancer

Enhancer ID: E_01_0512
Species: human
Position : chr13:40553262-40555262
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Nothing
Pubmed ID:  29735004
Enhancer experiment: Luciferase assay,Treatment with siRNAs,RT-qPCR,Immunoblotting,Immunofluorescence,transfection,
Enhancer experiment description: EPAC2 knockdown markedly reduced Phe-stimulated C/EBP? and C/EBP? mRNA levels, as well as forkhead box O1 (FOXO1) protein levels. These results suggest that EPAC signalling enhances PKA-mediated dPRL expression in ESCs by acting on C/EBP response elements in the promoter region of the gene.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: EPAC2 knockdown markedly reduced Phe-stimulated C/EBP? and C/EBP? mRNA levels, as well as forkhead box O1 (FOXO1) protein levels. These results suggest that EPAC signalling enhances PKA-mediated dPRL expression in ESCs by acting on C/EBP response elements in the promoter region of the gene.

About TF

TF name : FOXO1(FKH1,FKHRA,FOXO1)
TF experiment: Luciferase assay,Treatment with siRNAs,RT-qPCR,Immunoblotting,Immunofluorescence,transfection,
TF experiment description: EPAC2 knockdown markedly reduced Phe-stimulated C/EBP? and C/EBP? mRNA levels, as well as forkhead box O1 (FOXO1) protein levels. These results suggest that EPAC signalling enhances PKA-mediated dPRL expression in ESCs by acting on C/EBP response elements in the promoter region of the gene.

About Function

Enhancer function : EPAC2 knockdown markedly reduced Phe-stimulated C/EBP? and C/EBP? mRNA levels, as well as forkhead box O1 (FOXO1) protein levels. These results suggest that EPAC signalling enhances PKA-mediated dPRL expression in ESCs by acting on C/EBP response elements in the promoter region of the gene.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 EPAC2 knockdown markedly reduced Phe-stimulated C/EBP? and C/EBP? mRNA levels, as well as forkhead box O1 (FOXO1) protein levels. These results suggest that EPAC signalling enhances PKA-mediated dPRL expression in ESCs by acting on C/EBP response elements in the promoter region of the gene.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
FOXO1 AKT phosphorylates targets in the nucleus reactome 9
FOXO1 AndrogenReceptor netpath 167
FOXO1 Angiopoietin receptor Tie2-mediated signaling pid 50
FOXO1 BCR netpath 161
FOXO1 Class I PI3K signaling events mediated by Akt pid 35
FOXO1 Constitutive Signaling by AKT1 E17K in Cancer reactome 25
FOXO1 CXCR4-mediated signaling events pid 87
FOXO1 EGFR1 netpath 475
FOXO1 FoxO family signaling pid 50
FOXO1 FSH netpath 44
FOXO1 IL-7 netpath 28
FOXO1 IL3 netpath 84
FOXO1 IL6-mediated signaling events pid 48
FOXO1 IL6 netpath 85
FOXO1 Insulin/IGF pathway-protein kinase B signaling cascade panther 22
FOXO1 Leptin netpath 98
FOXO1 MAPK6/MAPK4 signaling reactome 90
FOXO1 Negative regulation of gene expression ( Insulin receptor signaling (D. melanogaster) ) inoh 7
FOXO1 Negative regulation of gene expression ( Insulin receptor signaling (Mammal) ) inoh 7
FOXO1 PI3 kinase pathway panther 38
FOXO1 Regulation of Androgen receptor activity pid 54
FOXO1 Regulation of gene expression in beta cells reactome 17
FOXO1 Regulation of nuclear SMAD2/3 signaling pid 82
FOXO1 Signaling events mediated by HDAC Class III pid 41
FOXO1 TGF_beta_Receptor netpath 220
FOXO1 TNFalpha netpath 274
FOXO1 Insulin signaling pathway kegg 134
FOXO1 Pathways in cancer kegg 321
FOXO1 Prostate cancer kegg 85
FOXO1 Hs_ErbB_Signaling_Pathway_WP673_91869 wikipathways 37
FOXO1 Hs_Angiopoietin_Like_Protein_8_Regulatory_Pathway_WP3915_90629 wikipathways 9
FOXO1 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
FOXO1 Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 wikipathways 153
FOXO1 Hs_Rac1-Pak1-p38-MMP-2_pathway_WP3303_87628 wikipathways 32
FOXO1 Hs_Energy_Metabolism_WP1541_91584 wikipathways 13
FOXO1 Hs_Transcription_factor_regulation_in_adipogenesis_WP3599_88581 wikipathways 9
FOXO1 Hs_NAD_metabolism,_sirtuins_and_aging_WP3630_88178 wikipathways 8
FOXO1 Hs_AGE-RAGE_pathway_WP2324_89798 wikipathways 27
FOXO1 Hs_Follicle_Stimulating_Hormone_(FSH)_signaling_pathway_WP2035_86080 wikipathways 20

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs