About Enhancer

Enhancer ID: E_01_0513
Species: human
Position : chr6:108557419-108559419
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Nothing
Pubmed ID:  29733381
Enhancer experiment: CRISPR/Cas9,Quantitative real-time PCR,Immunoblotting,Cell transfection,RNA interference,Cloning,ChIP,Luciferase assay,ChIP-loop assay,Cellular glutathione assay,DNA damage assay,Cell proliferation assay,
Enhancer experiment description: Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.

About Target gene

Target gene : SOD2(IPO-B,IPOB,MNSOD,MVCD6,Mn-SOD)
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.;Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.;Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.;Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.

About TF

TF name : FOXO3(1110048B16Rik,2010203A17Rik,C76856,FKHRL1,Fkhr2a,Foxo3)HSF1(HSTF1)GADD45A
TF experiment: CRISPR/Cas9,Quantitative real-time PCR,Immunoblotting,Cell transfection,RNA interference,Cloning,ChIP,Luciferase assay,ChIP-loop assay,Cellular glutathione assay,DNA damage assay,Cell proliferation assay,
TF experiment description: Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.;Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.;Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.;Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.

About Function

Enhancer function : Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 Our functional studies highlighted the importance of the HSF1-FOXO3-SOD2/CAT/GADD45A cascade in cellular stress response and survival by promoting ROS detoxification, redox balance and DNA repair. Our findings suggest the existence of an HSF1-FOXO3 axis in human cells that could be involved in stress response pathways functionally regulating lifespan and disease susceptibility.

About SNP

SNP ID: rs2802292

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
FOXO3 AKT phosphorylates targets in the nucleus reactome 9
FOXO3 BCR netpath 161
FOXO3 Class I PI3K signaling events pid 46
FOXO3 Class I PI3K signaling events mediated by Akt pid 35
FOXO3 Constitutive Signaling by AKT1 E17K in Cancer reactome 25
FOXO3 FoxO family signaling pid 50
FOXO3 FSH netpath 44
FOXO3 IL-7 netpath 28
FOXO3 IL2 netpath 81
FOXO3 IL2 signaling events mediated by PI3K pid 38
FOXO3 IL3 netpath 84
FOXO3 IL5 netpath 59
FOXO3 IL6 netpath 85
FOXO3 Insulin Pathway pid 47
FOXO3 Insulin/IGF pathway-protein kinase B signaling cascade panther 22
FOXO3 Interleukin signaling pathway panther 86
FOXO3 KitReceptor netpath 104
FOXO3 MAPK6/MAPK4 signaling reactome 90
FOXO3 Negative regulation of gene expression ( Insulin receptor signaling (D. melanogaster) ) inoh 7
FOXO3 Negative regulation of gene expression ( Insulin receptor signaling (Mammal) ) inoh 7
FOXO3 p73 transcription factor network pid 80
FOXO3 PI3 kinase pathway panther 38
FOXO3 Prolactin netpath 105
FOXO3 Regulation of nuclear SMAD2/3 signaling pid 82
FOXO3 Signaling by NODAL reactome 19
FOXO3 Signaling events mediated by HDAC Class III pid 41
FOXO3 Signaling events mediated by Stem cell factor receptor (c-Kit) pid 53
FOXO3 TGF_beta_Receptor netpath 220
FOXO3 Trk receptor signaling mediated by PI3K and PLC-gamma pid 36
FOXO3 Validated targets of C-MYC transcriptional repression pid 63
FOXO3 Chemokine signaling pathway kegg 187
FOXO3 Neurotrophin signaling pathway kegg 126
FOXO3 Endometrial cancer kegg 52
FOXO3 Non-small cell lung cancer kegg 54
FOXO3 Hs_DNA_Damage_Response_(only_ATM_dependent)_WP710_79974 wikipathways 36
FOXO3 Hs_Imatinib_and_Chronic_Myeloid_Leukemia_WP3640_89384 wikipathways 11
FOXO3 Hs_Endochondral_Ossification_WP474_87977 wikipathways 43
FOXO3 Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 wikipathways 153
FOXO3 Hs_Pathways_Affected_in_Adenoid_Cystic_Carcinoma_WP3651_89271 wikipathways 27
FOXO3 Hs_Energy_Metabolism_WP1541_91584 wikipathways 13
FOXO3 Hs_Estrogen_signaling_pathway_WP712_78491 wikipathways 13
FOXO3 Hs_IL-2_Signaling_Pathway_WP49_91243 wikipathways 33
FOXO3 Hs_NAD_metabolism,_sirtuins_and_aging_WP3630_88178 wikipathways 8
FOXO3 Hs_Brain-Derived_Neurotrophic_Factor_(BDNF)_signaling_pathway_WP2380_89803 wikipathways 80
HSF1 Attenuation phase reactome 28
HSF1 HSF1-dependent transactivation reactome 34
HSF1 HSF1 activation reactome 31
HSF1 p73 transcription factor network pid 80
HSF1 Regulation of HSF1-mediated heat shock response reactome 82
HSF1 Hs_DNA_IR-Double_Strand_Breaks_(DSBs)_and_cellular_response_via_ATM_WP3959_91367 wikipathways 50
GADD45A ATF-2 transcription factor network pid 59
GADD45A Aurora A signaling pid 33
GADD45A Direct p53 effectors pid 141
GADD45A FoxO family signaling pid 50
GADD45A p38 MAPK pathway panther 31
GADD45A p38 MAPK signaling pathway pid 29
GADD45A p53 pathway panther 39
GADD45A TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest reactome 18
GADD45A Validated targets of C-MYC transcriptional repression pid 63
GADD45A Validated transcriptional targets of TAp63 isoforms pid 54
GADD45A MAPK signaling pathway kegg 264
GADD45A Cell cycle kegg 124
GADD45A p53 signaling pathway kegg 66
GADD45A Hs_Imatinib_and_Chronic_Myeloid_Leukemia_WP3640_89384 wikipathways 11
GADD45A Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
GADD45A Hs_ATM_Signaling_Pathway_WP2516_90247 wikipathways 32
GADD45A Hs_TGF-beta_Receptor_Signaling_WP560_90187 wikipathways 28
GADD45A Hs_Oxidative_Damage_WP3941_90810 wikipathways 12
GADD45A Hs_G1_to_S_cell_cycle_control_WP45_80001 wikipathways 8

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs