About Enhancer

Enhancer ID: E_01_0514
Species: human
Position : chr7:148804423-148806423
Biosample name:
Experiment class : High+Lowthroughput
Enhancer type: Enhancer
Disease: Conjunctival malignant melanoma (cm)
Pubmed ID:  29732557
Enhancer experiment: Immunohistochemistry (IHC) staining,Immunoblotting,Cell proliferation,colony assay,Flow cytometry,RNA isolation,cDNA synthesis,quantitative PCR,
Enhancer experiment description: Our results indicate that elevated levels of EZH2 are relevant to CM tumourigenesis and progression, and that EZH2 may become a potential therapeutic target for patients with CM.

About Target gene

Target gene : --
Strong evidence: qRT-PCR,qPCR,ChIP,3C
Less strong evidence: RNA-Seq
Target gene experiment description: Our results indicate that elevated levels of EZH2 are relevant to CM tumourigenesis and progression, and that EZH2 may become a potential therapeutic target for patients with CM.;Our results indicate that elevated levels of EZH2 are relevant to CM tumourigenesis and progression, and that EZH2 may become a potential therapeutic target for patients with CM.

About TF

TF name : EZH2(ENX-1,ENX1b,KMT6,KMT6A,WVS,WVS2,EZH2)CDKN1A
TF experiment: Immunohistochemistry (IHC) staining,Immunoblotting,Cell proliferation,colony assay,Flow cytometry,RNA isolation,cDNA synthesis,quantitative PCR,
TF experiment description: Our results indicate that elevated levels of EZH2 are relevant to CM tumourigenesis and progression, and that EZH2 may become a potential therapeutic target for patients with CM.;Our results indicate that elevated levels of EZH2 are relevant to CM tumourigenesis and progression, and that EZH2 may become a potential therapeutic target for patients with CM.

About Function

Enhancer function : Our results indicate that elevated levels of EZH2 are relevant to CM tumourigenesis and progression, and that EZH2 may become a potential therapeutic target for patients with CM.
Enhancer function experiment: Immunohistochemical staining
Enhancer function
experiment description:		 Our results indicate that elevated levels of EZH2 are relevant to CM tumourigenesis and progression, and that EZH2 may become a potential therapeutic target for patients with CM.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
EZH2 Activation of anterior HOX genes in hindbrain development during early embryogenesis reactome 120
EZH2 Oxidative Stress Induced Senescence reactome 120
EZH2 PKMTs methylate histone lysines reactome 64
EZH2 PRC2 methylates histones and DNA reactome 71
EZH2 Hs_Endoderm_Differentiation_WP2853_88152 wikipathways 62
EZH2 Hs_Interactome_of_polycomb_repressive_complex_2_(PRC2)_WP2916_88672 wikipathways 15
CDKN1A AKT phosphorylates targets in the cytosol reactome 13
CDKN1A Angiopoietin receptor Tie2-mediated signaling pid 50
CDKN1A C-MYB transcription factor network pid 87
CDKN1A Ci/Gli processing signaling ( Hedgehog signaling pathway ) inoh 17
CDKN1A Class I PI3K signaling events mediated by Akt pid 35
CDKN1A Constitutive Signaling by AKT1 E17K in Cancer reactome 25
CDKN1A Cyclin A:Cdk2-associated events at S phase entry reactome 15
CDKN1A Cyclin D associated events in G1 reactome 38
CDKN1A Cyclin E associated events during G1/S transition reactome 14
CDKN1A Direct p53 effectors pid 141
CDKN1A DNA Damage/Telomere Stress Induced Senescence reactome 64
CDKN1A E2F transcription factor network pid 77
CDKN1A Formation of Senescence-Associated Heterochromatin Foci (SAHF) reactome 17
CDKN1A Gli processing signaling ( Hedgehog signaling pathway ) inoh 15
CDKN1A Glucocorticoid receptor regulatory network pid 85
CDKN1A Interleukin signaling pathway panther 86
CDKN1A Notch signaling pathway pid 58
CDKN1A Orc1 removal from chromatin reactome 70
CDKN1A p53-Dependent G1 DNA Damage Response reactome 7
CDKN1A p53 pathway panther 39
CDKN1A p53 pathway feedback loops 2 panther 42
CDKN1A p73 transcription factor network pid 80
CDKN1A Regulation of nuclear SMAD2/3 signaling pid 82
CDKN1A Regulation of retinoblastoma protein pid 67
CDKN1A SCF(Skp2)-mediated degradation of p27/p21 reactome 59
CDKN1A Senescence-Associated Secretory Phenotype (SASP) reactome 108
CDKN1A Signaling events mediated by HDAC Class III pid 41
CDKN1A Signaling events mediated by PRL pid 23
CDKN1A TGF_beta_Receptor netpath 220
CDKN1A The role of GTSE1 in G2/M progression after G2 checkpoint reactome 59
CDKN1A TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest reactome 13
CDKN1A Validated targets of C-MYC transcriptional repression pid 63
CDKN1A Validated transcriptional targets of TAp63 isoforms pid 54
CDKN1A Vinblastine Action Pathway smpdb 11
CDKN1A Vincristine Action Pathway smpdb 10
CDKN1A Vindesine Action Pathway smpdb 10
CDKN1A Vinorelbine Action Pathway smpdb 11
CDKN1A ErbB signaling pathway kegg 83
CDKN1A Cell cycle kegg 124
CDKN1A p53 signaling pathway kegg 66
CDKN1A Hepatitis C kegg 134
CDKN1A Pathways in cancer kegg 321
CDKN1A Glioma kegg 65
CDKN1A Prostate cancer kegg 85
CDKN1A Melanoma kegg 69
CDKN1A Bladder cancer kegg 38
CDKN1A Chronic myeloid leukemia kegg 69
CDKN1A Hs_Cell_Cycle_WP179_89516 wikipathways 33
CDKN1A Hs_ErbB_Signaling_Pathway_WP673_91869 wikipathways 37
CDKN1A Hs_DNA_Damage_Response_(only_ATM_dependent)_WP710_79974 wikipathways 36
CDKN1A Hs_DNA_Damage_Response_WP707_82937 wikipathways 28
CDKN1A Hs_miRNA_Regulation_of_DNA_Damage_Response_WP1530_84694 wikipathways 28
CDKN1A Hs_Apoptosis-related_network_due_to_altered_Notch3_in_ovarian_cancer_WP2864_79278 wikipathways 51
CDKN1A Hs_miRNAs_involved_in_DNA_damage_response_WP1545_84697 wikipathways 30
CDKN1A Hs_Hepatitis_C_and_Hepatocellular_Carcinoma_WP3646_88640 wikipathways 36
CDKN1A Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193 wikipathways 62
CDKN1A Hs_Integrated_Cancer_Pathway_WP1971_82939 wikipathways 33
CDKN1A Hs_ATM_Signaling_Pathway_WP2516_90247 wikipathways 32
CDKN1A Hs_LncRNA-mediated_mechanisms_of_therapeutic_resistance_WP3672_87395 wikipathways 9
CDKN1A Hs_Signaling_Pathways_in_Glioblastoma_WP2261_89520 wikipathways 16
CDKN1A Hs_AMP-activated_Protein_Kinase_(AMPK)_Signaling_WP1403_90259 wikipathways 11
CDKN1A Hs_Aryl_Hydrocarbon_Receptor_WP2586_91687 wikipathways 16
CDKN1A Hs_Retinoblastoma_(RB)_in_Cancer_WP2446_87639 wikipathways 5
CDKN1A Hs_TGF-beta_Signaling_Pathway_WP366_90028 wikipathways 67
CDKN1A Hs_Androgen_receptor_signaling_pathway_WP138_79958 wikipathways 27

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs