About Enhancer
| Enhancer ID: | E_01_0614 |
| Species: | human |
| Position : | chr22:39516997-39518997   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Diabetic cardiomyopathy |
| Pubmed ID: | 32566584 |
| Enhancer experiment: | flow cytometry?Western blotting?Enzyme-linked immunosorbent assay |
| Enhancer experiment description: | Results: BC significantly inhibited AGE-induced cell death and apoptosis in H9c2 cells. BC had a suppressive effect on intracellular ROS production and antioxidative enzyme reduction. Moreover, BC decreased hyperactive endoplasmic reticulum (ER) stress and autophagy in H9c2 cells. Furthermore, BC exerted a cardioprotective effect in AGE-induced H9c2 cells via the activation of the PI3K/Akt/mTOR signaling pathway. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Results: BC significantly inhibited AGE-induced cell death and apoptosis in H9c2 cells. BC had a suppressive effect on intracellular ROS production and antioxidative enzyme reduction. Moreover, BC decreased hyperactive endoplasmic reticulum (ER) stress and autophagy in H9c2 cells. Furthermore, BC exerted a cardioprotective effect in AGE-induced H9c2 cells via the activation of the PI3K/Akt/mTOR signaling pathway. |
About TF
| TF name : | ATF4 |
| TF experiment: | flow cytometry?Western blotting?Enzyme-linked immunosorbent assay |
| TF experiment description: | Results: BC significantly inhibited AGE-induced cell death and apoptosis in H9c2 cells. BC had a suppressive effect on intracellular ROS production and antioxidative enzyme reduction. Moreover, BC decreased hyperactive endoplasmic reticulum (ER) stress and autophagy in H9c2 cells. Furthermore, BC exerted a cardioprotective effect in AGE-induced H9c2 cells via the activation of the PI3K/Akt/mTOR signaling pathway. |
About Function
| Enhancer function : | Results: BC significantly inhibited AGE-induced cell death and apoptosis in H9c2 cells. BC had a suppressive effect on intracellular ROS production and antioxidative enzyme reduction. Moreover, BC decreased hyperactive endoplasmic reticulum (ER) stress and autophagy in H9c2 cells. Furthermore, BC exerted a cardioprotective effect in AGE-induced H9c2 cells via the activation of the PI3K/Akt/mTOR signaling pathway. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Results: BC significantly inhibited AGE-induced cell death and apoptosis in H9c2 cells. BC had a suppressive effect on intracellular ROS production and antioxidative enzyme reduction. Moreover, BC decreased hyperactive endoplasmic reticulum (ER) stress and autophagy in H9c2 cells. Furthermore, BC exerted a cardioprotective effect in AGE-induced H9c2 cells via the activation of the PI3K/Akt/mTOR signaling pathway. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| ATF4 | Apoptosis signaling pathway | panther | 104 |
| ATF4 | ATF4 activates genes | reactome | 25 |
| ATF4 | ATF6-alpha activates chaperone genes | reactome | 10 |
| ATF4 | Validated transcriptional targets of AP1 family members Fra1 and Fra2 | pid | 37 |
| ATF4 | MAPK signaling pathway | kegg | 264 |
| ATF4 | Protein processing in endoplasmic reticulum | kegg | 166 |
| ATF4 | Long-term potentiation | kegg | 67 |
| ATF4 | Neurotrophin signaling pathway | kegg | 126 |
| ATF4 | GnRH signaling pathway | kegg | 98 |
| ATF4 | Prostate cancer | kegg | 85 |
| ATF4 | Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 | wikipathways | 153 |
| ATF4 | Hs_White_fat_cell_differentiation_WP3946_90940 | wikipathways | 30 |
| ATF4 | Hs_One_Carbon_Metabolism_WP241_89671 | wikipathways | 14 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene