About Enhancer
| Enhancer ID: | E_01_0635 |
| Species: | human |
| Position : | chr11:75397365-75399365   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Nothing |
| Pubmed ID: | 30428538 |
| Enhancer experiment: | Protein purification,Cell culture and transfection,Co-immunoprecipitation assay,Mass Spectrometry,RNA interference and transfection, |
| Enhancer experiment description: | The EHEC NleH1 effector inhibits the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) pathway by reducing the nuclear translocation of the ribosomal protein S3 (RPS3). NleH1 prevents RPS3 phosphorylation by the I?B kinase-? (IKK?). IKK? is a central kinase in the NF-?B pathway, yet NleH1 only restricts the phosphorylation of a subset of the IKK? substrates. We hypothesized that a protein cofactor might dictate this inhibitory specificity. |
About Target gene
| Target gene : | RPS3 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | The EHEC NleH1 effector inhibits the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) pathway by reducing the nuclear translocation of the ribosomal protein S3 (RPS3). NleH1 prevents RPS3 phosphorylation by the I?B kinase-? (IKK?). IKK? is a central kinase in the NF-?B pathway, yet NleH1 only restricts the phosphorylation of a subset of the IKK? substrates. We hypothesized that a protein cofactor might dictate this inhibitory specificity. |
About TF
| TF name : | -- |
| TF experiment: | Protein purification,Cell culture and transfection,Co-immunoprecipitation assay,Mass Spectrometry,RNA interference and transfection, |
| TF experiment description: | The EHEC NleH1 effector inhibits the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) pathway by reducing the nuclear translocation of the ribosomal protein S3 (RPS3). NleH1 prevents RPS3 phosphorylation by the I?B kinase-? (IKK?). IKK? is a central kinase in the NF-?B pathway, yet NleH1 only restricts the phosphorylation of a subset of the IKK? substrates. We hypothesized that a protein cofactor might dictate this inhibitory specificity. |
About Function
| Enhancer function : | The EHEC NleH1 effector inhibits the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) pathway by reducing the nuclear translocation of the ribosomal protein S3 (RPS3). NleH1 prevents RPS3 phosphorylation by the I?B kinase-? (IKK?). IKK? is a central kinase in the NF-?B pathway, yet NleH1 only restricts the phosphorylation of a subset of the IKK? substrates. We hypothesized that a protein cofactor might dictate this inhibitory specificity. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
The EHEC NleH1 effector inhibits the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) pathway by reducing the nuclear translocation of the ribosomal protein S3 (RPS3). NleH1 prevents RPS3 phosphorylation by the I?B kinase-? (IKK?). IKK? is a central kinase in the NF-?B pathway, yet NleH1 only restricts the phosphorylation of a subset of the IKK? substrates. We hypothesized that a protein cofactor might dictate this inhibitory specificity. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene