About Enhancer
| Enhancer ID: | E_01_0665 |
| Species: | human |
| Position : | chr22:39516937-39518937   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Gallbladder cancer |
| Pubmed ID: | 30344724 |
| Enhancer experiment: | flow cytometry, western blot analysis, ELISA and reverse transcription-quantitative polymerase chain reaction (RT-qPCR),siRNA sequences synthesis, |
| Enhancer experiment description: | It was indicated that Rg3 exerted pro-apoptotic activity against the gallbladder cancer cell line GBC-SD through the ER stress-mediated signaling pathway. This was demonstrated by increased expression of phosphorylation of eukaryotic translation-initiation factor 2? (eIF2?), activating transcription factor 4 (ATF4), CCAAT/enhancer-binding protein homologous protein and lipocalin 2. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | It was indicated that Rg3 exerted pro-apoptotic activity against the gallbladder cancer cell line GBC-SD through the ER stress-mediated signaling pathway. This was demonstrated by increased expression of phosphorylation of eukaryotic translation-initiation factor 2? (eIF2?), activating transcription factor 4 (ATF4), CCAAT/enhancer-binding protein homologous protein and lipocalin 2. |
About TF
| TF name : | ATF4 |
| TF experiment: | flow cytometry, western blot analysis, ELISA and reverse transcription-quantitative polymerase chain reaction (RT-qPCR),siRNA sequences synthesis, |
| TF experiment description: | It was indicated that Rg3 exerted pro-apoptotic activity against the gallbladder cancer cell line GBC-SD through the ER stress-mediated signaling pathway. This was demonstrated by increased expression of phosphorylation of eukaryotic translation-initiation factor 2? (eIF2?), activating transcription factor 4 (ATF4), CCAAT/enhancer-binding protein homologous protein and lipocalin 2. |
About Function
| Enhancer function : | It was indicated that Rg3 exerted pro-apoptotic activity against the gallbladder cancer cell line GBC-SD through the ER stress-mediated signaling pathway. This was demonstrated by increased expression of phosphorylation of eukaryotic translation-initiation factor 2? (eIF2?), activating transcription factor 4 (ATF4), CCAAT/enhancer-binding protein homologous protein and lipocalin 2. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
It was indicated that Rg3 exerted pro-apoptotic activity against the gallbladder cancer cell line GBC-SD through the ER stress-mediated signaling pathway. This was demonstrated by increased expression of phosphorylation of eukaryotic translation-initiation factor 2? (eIF2?), activating transcription factor 4 (ATF4), CCAAT/enhancer-binding protein homologous protein and lipocalin 2. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| ATF4 | Apoptosis signaling pathway | panther | 104 |
| ATF4 | ATF4 activates genes | reactome | 25 |
| ATF4 | ATF6-alpha activates chaperone genes | reactome | 10 |
| ATF4 | Validated transcriptional targets of AP1 family members Fra1 and Fra2 | pid | 37 |
| ATF4 | MAPK signaling pathway | kegg | 264 |
| ATF4 | Protein processing in endoplasmic reticulum | kegg | 166 |
| ATF4 | Long-term potentiation | kegg | 67 |
| ATF4 | Neurotrophin signaling pathway | kegg | 126 |
| ATF4 | GnRH signaling pathway | kegg | 98 |
| ATF4 | Prostate cancer | kegg | 85 |
| ATF4 | Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 | wikipathways | 153 |
| ATF4 | Hs_White_fat_cell_differentiation_WP3946_90940 | wikipathways | 30 |
| ATF4 | Hs_One_Carbon_Metabolism_WP241_89671 | wikipathways | 14 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene