About Enhancer
| Enhancer ID: | E_01_0744 |
| Species: | human |
| Position : | chr12:53959572-53961572   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Gastrointestinal cancer, hepatocellular carcinoma |
| Pubmed ID: | 30154449 |
| Enhancer experiment: | RT qPCR, chromatin immunoprecipitation (chip) analysis, statistical analysis, Weston blot |
| Enhancer experiment description: | Furthermore, functional studies revealed a pivotal role for HOTAIR in the epithelial-to-mesenchymal transition, as this RNA is causal for the repressive activity of the master factor SNAIL on epithelial genes. We recently demonstrated that HOTAIR expression is induced in hepatocytes undergoing EMT and functions to bridge, in specific chromatin sites, the interaction between EZH2 and Snail. In other words, the EMT master factor Snail (i.e., sufficient to trigger and orchestrate the transition) conveys the Polycomb catalytic subunit to specific sites by means of a direct interaction with HOTAIR. |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Furthermore, functional studies revealed a pivotal role for HOTAIR in the epithelial-to-mesenchymal transition, as this RNA is causal for the repressive activity of the master factor SNAIL on epithelial genes. We recently demonstrated that HOTAIR expression is induced in hepatocytes undergoing EMT and functions to bridge, in specific chromatin sites, the interaction between EZH2 and Snail. In other words, the EMT master factor Snail (i.e., sufficient to trigger and orchestrate the transition) conveys the Polycomb catalytic subunit to specific sites by means of a direct interaction with HOTAIR.;Furthermore, functional studies revealed a pivotal role for HOTAIR in the epithelial-to-mesenchymal transition, as this RNA is causal for the repressive activity of the master factor SNAIL on epithelial genes. We recently demonstrated that HOTAIR expression is induced in hepatocytes undergoing EMT and functions to bridge, in specific chromatin sites, the interaction between EZH2 and Snail. In other words, the EMT master factor Snail (i.e., sufficient to trigger and orchestrate the transition) conveys the Polycomb catalytic subunit to specific sites by means of a direct interaction with HOTAIR. |
About TF
| TF name : | HOTAIREZH2(ENX-1,ENX1b,KMT6,KMT6A,WVS,WVS2,EZH2) |
| TF experiment: | RT-qPCR,Chromatin immunoprecipitation (ChIP) analysis,????,weston blot |
| TF experiment description: | Furthermore, functional studies revealed a pivotal role for HOTAIR in the epithelial-to-mesenchymal transition, as this RNA is causal for the repressive activity of the master factor SNAIL on epithelial genes. We recently demonstrated that HOTAIR expression is induced in hepatocytes undergoing EMT and functions to bridge, in specific chromatin sites, the interaction between EZH2 and Snail. In other words, the EMT master factor Snail (i.e., sufficient to trigger and orchestrate the transition) conveys the Polycomb catalytic subunit to specific sites by means of a direct interaction with HOTAIR.;Furthermore, functional studies revealed a pivotal role for HOTAIR in the epithelial-to-mesenchymal transition, as this RNA is causal for the repressive activity of the master factor SNAIL on epithelial genes. We recently demonstrated that HOTAIR expression is induced in hepatocytes undergoing EMT and functions to bridge, in specific chromatin sites, the interaction between EZH2 and Snail. In other words, the EMT master factor Snail (i.e., sufficient to trigger and orchestrate the transition) conveys the Polycomb catalytic subunit to specific sites by means of a direct interaction with HOTAIR. |
About Function
| Enhancer function : | Furthermore, functional studies revealed a pivotal role for HOTAIR in the epithelial-to-mesenchymal transition, as this RNA is causal for the repressive activity of the master factor SNAIL on epithelial genes. We recently demonstrated that HOTAIR expression is induced in hepatocytes undergoing EMT and functions to bridge, in specific chromatin sites, the interaction between EZH2 and Snail. In other words, the EMT master factor Snail (i.e., sufficient to trigger and orchestrate the transition) conveys the Polycomb catalytic subunit to specific sites by means of a direct interaction with HOTAIR. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Furthermore, functional studies revealed a pivotal role for HOTAIR in the epithelial-to-mesenchymal transition, as this RNA is causal for the repressive activity of the master factor SNAIL on epithelial genes. We recently demonstrated that HOTAIR expression is induced in hepatocytes undergoing EMT and functions to bridge, in specific chromatin sites, the interaction between EZH2 and Snail. In other words, the EMT master factor Snail (i.e., sufficient to trigger and orchestrate the transition) conveys the Polycomb catalytic subunit to specific sites by means of a direct interaction with HOTAIR. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| HOTAIR | Hs_LncRNA-mediated_mechanisms_of_therapeutic_resistance_WP3672_87395 | wikipathways | 9 |
| EZH2 | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
| EZH2 | Oxidative Stress Induced Senescence | reactome | 120 |
| EZH2 | PKMTs methylate histone lysines | reactome | 64 |
| EZH2 | PRC2 methylates histones and DNA | reactome | 71 |
| EZH2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| EZH2 | Hs_Interactome_of_polycomb_repressive_complex_2_(PRC2)_WP2916_88672 | wikipathways | 15 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene