About Enhancer
| Enhancer ID: | E_01_0959 |
| Species: | human |
| Position : | chr6:45325376-45327376   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Arthritis |
| Pubmed ID: | 32632701 |
| Enhancer experiment: | Flow cytometry,Western blot |
| Enhancer experiment description: | The signifcant loss of chondrocytes in grades 2 and 3 of LFJs was observed. The expression levels of CCAAT enhancer binding protein ? (C/EBP?), Runt-related transcription factor 2 (RUNX2), and matrix metalloproteinase 13 (MMP13) also increased with the aggravation of degeneration (4.89, 5.77, and 6.3 times by Western blot). |
About Target gene
| Target gene : | -- |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | The signifcant loss of chondrocytes in grades 2 and 3 of LFJs was observed. The expression levels of CCAAT enhancer binding protein ? (C/EBP?), Runt-related transcription factor 2 (RUNX2), and matrix metalloproteinase 13 (MMP13) also increased with the aggravation of degeneration (4.89, 5.77, and 6.3 times by Western blot). |
About TF
| TF name : | RUNX2(AML3,Cbf,Cbfa-1,Cbfa1,LS3,Osf2,PEBP2aA,Pebp2a1,Pebpa2a) |
| TF experiment: | Flow cytometry,Western blot |
| TF experiment description: | The signifcant loss of chondrocytes in grades 2 and 3 of LFJs was observed. The expression levels of CCAAT enhancer binding protein ? (C/EBP?), Runt-related transcription factor 2 (RUNX2), and matrix metalloproteinase 13 (MMP13) also increased with the aggravation of degeneration (4.89, 5.77, and 6.3 times by Western blot). |
About Function
| Enhancer function : | The signifcant loss of chondrocytes in grades 2 and 3 of LFJs was observed. The expression levels of CCAAT enhancer binding protein ? (C/EBP?), Runt-related transcription factor 2 (RUNX2), and matrix metalloproteinase 13 (MMP13) also increased with the aggravation of degeneration (4.89, 5.77, and 6.3 times by Western blot). |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
The signifcant loss of chondrocytes in grades 2 and 3 of LFJs was observed. The expression levels of CCAAT enhancer binding protein ? (C/EBP?), Runt-related transcription factor 2 (RUNX2), and matrix metalloproteinase 13 (MMP13) also increased with the aggravation of degeneration (4.89, 5.77, and 6.3 times by Western blot). |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| RUNX2 | AndrogenReceptor | netpath | 167 |
| RUNX2 | FGF signaling pathway | pid | 55 |
| RUNX2 | Leptin | netpath | 98 |
| RUNX2 | Notch-mediated HES/HEY network | pid | 48 |
| RUNX2 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| RUNX2 | Regulation of retinoblastoma protein | pid | 67 |
| RUNX2 | TGF_beta_Receptor | netpath | 220 |
| RUNX2 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| RUNX2 | Hs_MAPK_Signaling_Pathway_WP382_79951 | wikipathways | 58 |
| RUNX2 | Hs_Endochondral_Ossification_WP474_87977 | wikipathways | 43 |
| RUNX2 | Hs_Role_of_Osx_and_miRNAs_in_tooth_development_WP3971_91525 | wikipathways | 9 |
| RUNX2 | Hs_Bone_Morphogenic_Protein_(BMP)_Signalling_and_Regulation_WP1425_86082 | wikipathways | 6 |
| RUNX2 | Hs_Focal_Adhesion-PI3K-Akt-mTOR-signaling_pathway_WP3932_91101 | wikipathways | 8 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene