About Enhancer
| Enhancer ID: | E_02_0008 |
| Species: | mouse |
| Position : | chr15:63714919-63952099   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | Leukaemia |
| Pubmed ID: | 29342133 |
| Enhancer experiment: | ChIP-seq |
| Enhancer experiment description: | Here we show that an evolutionarily conserved region located 1.7 megabases downstream of the Myc gene that has previously been labelled as a super_x0002_enhancer2 is essential for the regulation of Myc expression levels in both normal haematopoietic and leukaemic stem cell hierarchies in mice and humans. Deletion of this region in mice leads to a complete loss of Myc expression in haematopoietic stem cells and progenitors. |
About Target gene
| Target gene : | Myc(MRTLC,bHLHe39,c-Myc) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq,ATAC-seq,RT-qPCR |
| Target gene experiment description: | In line with this hypothesis, chromatin conformation capture performedon mouse leukaemic cells2 as well as DNA proximity analysis using fluorescence in situ hybridization (DNA FISH) in LSK cells showed that the Myc promoter and BENC are in close physical proximity to each other, suggesting that cis-regulation is mediated by chromosomal looping in haematopoietic stem and progenitor cells. |
About TF
| TF name : | -- |
| TF experiment: | -- |
| TF experiment description: | -- |
About Function
| Enhancer function : | Clusters of enhancers, such as BENC, form highly combinatorial systems that allow precise control of gene expression across normal cellular hierarchies and which also can be hijacked in malignancies. |
| Enhancer function experiment: | ATAC-seq,RT-qPCR |
| Enhancer function experiment description: |
Analysis of mice carrying deletions of individual Enhancer modules suggests that specific Myc expression levels throughout most of the haematopoietic hierarchy are controlled by the combinatorial and additive activity of individual Enhancer modules, which collectively function as a blood Enhancer cluster(BENC). |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene