About Enhancer
| Enhancer ID: | E_02_0282 |
| Species: | mouse |
| Position : | chr11:112217210-112217767   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 29903884 |
| Enhancer experiment: | DNaseI-seq,ChIP-seq |
| Enhancer experiment description: | In vivo high-throughput chromatin accessibility techniques, transgenic assays, and genome editing revealed several novel gonadal regulatory elements in the 2 Mb gene desert upstream of Sox9.Although others are redundant, Enh13, a 557 bp element located 565 kb 5, is essential to initiate mouse testis development. |
About Target gene
| Target gene : | Sox9(CMD1,CMPD1,SRA1,SRXX2,SRXY10),Sox9(CMD1,CMPD1,SRA1,SRXX2,SRXY10),Sox9(CMD1,CMPD1,SRA1,SRXX2,SRXY10),Sox9(CMD1,CMPD1,SRA1,SRXX2,SRXY10) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | ChIP-seq,RT-PCR |
| Target gene experiment description: | There was a 30 to 50% decrease in Sox9 mRNA levels in E11.5 XX Enh13-/- gonads compared to the wild type, as reflected by reduced immunofluorescence for SOX9 protein (fig. S11). These data indicate that Enh13 also plays a role in the very early expression of Sox9 in the XX gonad.;There was a 30 to 50% decrease in Sox9 mRNA levels in E11.5 XX Enh13-/- gonads compared to the wild type, as reflected by reduced immunofluorescence for SOX9 protein (fig. S11). These data indicate that Enh13 also plays a role in the very early expression of Sox9 in the XX gonad.;There was a 30 to 50% decrease in Sox9 mRNA levels in E11.5 XX Enh13-/- gonads compared to the wild type, as reflected by reduced immunofluorescence for SOX9 protein (fig. S11). These data indicate that Enh13 also plays a role in the very early expression of Sox9 in the XX gonad.;There was a 30 to 50% decrease in Sox9 mRNA levels in E11.5 XX Enh13-/- gonads compared to the wild type, as reflected by reduced immunofluorescence for SOX9 protein (fig. S11). These data indicate that Enh13 also plays a role in the very early expression of Sox9 in the XX gonad. |
About TF
| TF name : | Sry(Tdf,Tdy)Sry(Tdf,Tdy)Sry(Tdf,Tdy)Sry(Tdf,Tdy) |
| TF experiment: | ChIP-qPCR |
| TF experiment description: | We performed ChIP-qPCR on E11.5 gonads dissected from Sry-Myc transgenic embryos by using a specific antibody against the MYC tag.SRY-MYCpositive gonads had an 11-fold enrichment versus SRY-MYCnegative gonads with primers spanning the SOX9 consensus site and a sixfold enrichment with primers spanning the SRY site, whereas primers against the strongest SRY binding site in TESCO showed fivefold enrichment.This reveals the strong binding of SRY to Enh13 at E11.5, with a preference for the SOX9 consensus site, possibly due to the adjacent SF1 binding site.;We performed ChIP-qPCR on E11.5 gonads dissected from Sry-Myc transgenic embryos by using a specific antibody against the MYC tag.SRY-MYCpositive gonads had an 11-fold enrichment versus SRY-MYCnegative gonads with primers spanning the SOX9 consensus site and a sixfold enrichment with primers spanning the SRY site, whereas primers against the strongest SRY binding site in TESCO showed fivefold enrichment.This reveals the strong binding of SRY to Enh13 at E11.5, with a preference for the SOX9 consensus site, possibly due to the adjacent SF1 binding site.;We performed ChIP-qPCR on E11.5 gonads dissected from Sry-Myc transgenic embryos by using a specific antibody against the MYC tag.SRY-MYCpositive gonads had an 11-fold enrichment versus SRY-MYCnegative gonads with primers spanning the SOX9 consensus site and a sixfold enrichment with primers spanning the SRY site, whereas primers against the strongest SRY binding site in TESCO showed fivefold enrichment.This reveals the strong binding of SRY to Enh13 at E11.5, with a preference for the SOX9 consensus site, possibly due to the adjacent SF1 binding site.;We performed ChIP-qPCR on E11.5 gonads dissected from Sry-Myc transgenic embryos by using a specific antibody against the MYC tag.SRY-MYCpositive gonads had an 11-fold enrichment versus SRY-MYCnegative gonads with primers spanning the SOX9 consensus site and a sixfold enrichment with primers spanning the SRY site, whereas primers against the strongest SRY binding site in TESCO showed fivefold enrichment.This reveals the strong binding of SRY to Enh13 at E11.5, with a preference for the SOX9 consensus site, possibly due to the adjacent SF1 binding site. |
About Function
| Enhancer function : | Enh13 is conserved and embedded within a 32.5-kilobase region whose deletion in humans is associated with XY sex reversal, suggesting that it is also critical in humans. |
| Enhancer function experiment: | CRISPR/Cas9,RT-qPCR |
| Enhancer function experiment description: |
This again supports the conclusion that Enh13 plays a more substantial role than TES during early gonadal development. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sry | AndrogenReceptor | netpath | 167 |
| Sry | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sry | Regulation of Androgen receptor activity | pid | 54 |
| Sry | AndrogenReceptor | netpath | 167 |
| Sry | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sry | Regulation of Androgen receptor activity | pid | 54 |
| Sry | AndrogenReceptor | netpath | 167 |
| Sry | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sry | Regulation of Androgen receptor activity | pid | 54 |
| Sry | AndrogenReceptor | netpath | 167 |
| Sry | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sry | Regulation of Androgen receptor activity | pid | 54 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene