About Enhancer
| Enhancer ID: | E_02_0283 |
| Species: | mouse |
| Position : | chr15:96725740-96737960   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Congenital heart disease |
| Pubmed ID: | 29903739 |
| Enhancer experiment: | ChIP-seq,Hi-C |
| Enhancer experiment description: | we made use of our TBX20 ChIP-Seq analysis in E11.5 hearts and attributed TBX20 bound sites to the nearest expressed gene in E11.5 control or Tbx20 cKO cardiomyocytes. Next, we overlaid our RNA-Seq data with that of our TBX20 ChIP-Seq analysis in E11.5 hearts and identified 548 genes that were differentially expressed in Tbx20 cKO cardiomyocytes and were marked by one or more TBX20 binding events in the vicinity of the gene. |
About Target gene
| Target gene : | NR2F2(ARP-1,ARP1,CHTD4,COUPTF2,COUPTFB,COUPTFII,NF-E3,SVP40,TFCOUP2),NR2F2(ARP-1,ARP1,CHTD4,COUPTF2,COUPTFB,COUPTFII,NF-E3,SVP40,TFCOUP2),NR2F2(ARP-1,ARP1,CHTD4,COUPTF2,COUPTFB,COUPTFII,NF-E3,SVP40,TFCOUP2) |
| Strong evidence: | -- |
| Less strong evidence: | Hi-C |
| Target gene experiment description: | We next established that this Enhancer was functionally connected with COUP-TFII. We performed a promoter-based Capture Hi-C in iPSC-derived human cardiomyocytes to identify long-range physical interactions between genes and Enhancers. We observed that this Enhancer directly loops and contacts the COUP-TFII promoter,140-Kb away,confirming that this is a COUP-TFII Enhancer.;We next established that this Enhancer was functionally connected with COUP-TFII. We performed a promoter-based Capture Hi-C in iPSC-derived human cardiomyocytes to identify long-range physical interactions between genes and Enhancers. We observed that this Enhancer directly loops and contacts the COUP-TFII promoter,140-Kb away,confirming that this is a COUP-TFII Enhancer.;We next established that this Enhancer was functionally connected with COUP-TFII. We performed a promoter-based Capture Hi-C in iPSC-derived human cardiomyocytes to identify long-range physical interactions between genes and Enhancers. We observed that this Enhancer directly loops and contacts the COUP-TFII promoter,140-Kb away,confirming that this is a COUP-TFII Enhancer. |
About TF
| TF name : | TBX20(ASD4)TBX20(ASD4)TBX20(ASD4) |
| TF experiment: | X-Gal Staining Assay |
| TF experiment description: | Section analysis of COUP-TFII enh1::lacZ transgenic embryo demonstrates X-gal staining in developing atrial myocardium (arrows) and caval vein (arrowheads). ;Section analysis of COUP-TFII enh1::lacZ transgenic embryo demonstrates X-gal staining in developing atrial myocardium (arrows) and caval vein (arrowheads). ;Section analysis of COUP-TFII enh1::lacZ transgenic embryo demonstrates X-gal staining in developing atrial myocardium (arrows) and caval vein (arrowheads). |
About Function
| Enhancer function : | This Enhancer interacted with the NR2F2 promoter in human cardiomyocytes and conferred atrial specific gene expression in a transgenic mouse in a TBX20 dependent manner. |
| Enhancer function experiment: | Hi-C |
| Enhancer function experiment description: |
Taken together, these results linked TBX20 binding to an evolutionary conserved Enhancer that regulates COUP-TFII expression in developing atrial cardiomyocytes, uncovering a mechanism by which COUP-TFII expression is TBX20 dependent. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| TBX20 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
| TBX20 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
| TBX20 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene