About Enhancer

Enhancer ID: E_02_0307
Species: human
Position : chr21:40003867-40015913
Biosample name:
Experiment class : Low+High throughput
Enhancer type: Super-Enhancer
Disease: Leukemia
Pubmed ID:  29765516
Enhancer experiment: ChIP-qPCR,ChIP-seq
Enhancer experiment description: Chromatin-immunoprecipitation followed by quantitative PCR (ChIP-qPCR) analysis for GFI1B and RUNX1 was performed at highly conserved noncoding areas including upstream, promotor, 3 UTR, and SE regions.

About Target gene

Target gene : ERG(erg-3,p55),ERG(erg-3,p55),ERG(erg-3,p55),ERG(erg-3,p55)
Strong evidence: --
Less strong evidence: ChIP-qPCR
Target gene experiment description: Furthermore,ChIP-qPCR analysis for H3K27ac revealed that the level of H3K27ac was elevated only at the SE region after the NCD38 treatment.;Furthermore,ChIP-qPCR analysis for H3K27ac revealed that the level of H3K27ac was elevated only at the SE region after the NCD38 treatment.;Furthermore,ChIP-qPCR analysis for H3K27ac revealed that the level of H3K27ac was elevated only at the SE region after the NCD38 treatment.;Furthermore,ChIP-qPCR analysis for H3K27ac revealed that the level of H3K27ac was elevated only at the SE region after the NCD38 treatment.

About TF

TF name : GFI1B(BDPLT17,ZNF163B)RUNX1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)KDM1A(AOF2,BHC110,CPRF,KDM1,LSD1)GFI1B(BDPLT17,ZNF163B)RUNX1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)KDM1A(AOF2,BHC110,CPRF,KDM1,LSD1)GFI1B(BDPLT17,ZNF163B)RUNX1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)KDM1A(AOF2,BHC110,CPRF,KDM1,LSD1)GFI1B(BDPLT17,ZNF163B)RUNX1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)KDM1A(AOF2,BHC110,CPRF,KDM1,LSD1)
TF experiment: ChIP-qPCR
TF experiment description: ChIP-qPCR analysis for GFI1B, RUNX1, or control IgG in the conserved non-coding regions at the ERG locus in HEL cells.ChIP-qPCR analysis for GFI1B (C), LSD1 (D), and CoREST (E) at the ERG-SE locus in HEL cells treated with DMSO or NCD38 for 24 hours. All ChIP-qPCR assays were performed independently twice and the means are displayed.;ChIP-qPCR analysis for GFI1B, RUNX1, or control IgG in the conserved non-coding regions at the ERG locus in HEL cells.ChIP-qPCR analysis for GFI1B (C), LSD1 (D), and CoREST (E) at the ERG-SE locus in HEL cells treated with DMSO or NCD38 for 24 hours. All ChIP-qPCR assays were performed independently twice and the means are displayed.;ChIP-qPCR analysis for GFI1B, RUNX1, or control IgG in the conserved non-coding regions at the ERG locus in HEL cells.ChIP-qPCR analysis for GFI1B (C), LSD1 (D), and CoREST (E) at the ERG-SE locus in HEL cells treated with DMSO or NCD38 for 24 hours. All ChIP-qPCR assays were performed independently twice and the means are displayed.;ChIP-qPCR analysis for GFI1B, RUNX1, or control IgG in the conserved non-coding regions at the ERG locus in HEL cells.ChIP-qPCR analysis for GFI1B (C), LSD1 (D), and CoREST (E) at the ERG-SE locus in HEL cells treated with DMSO or NCD38 for 24 hours. All ChIP-qPCR assays were performed independently twice and the means are displayed.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:		 --

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
RUNX1 AndrogenReceptor netpath 167
RUNX1 Organic cation transport reactome 9
RUNX1 RANKL netpath 84
RUNX1 Regulation of nuclear SMAD2/3 signaling pid 82
RUNX1 TGF_beta_Receptor netpath 220
RUNX1 Validated transcriptional targets of deltaNp63 isoforms pid 47
RUNX1 Pathways in cancer kegg 321
RUNX1 Chronic myeloid leukemia kegg 69
RUNX1 Acute myeloid leukemia kegg 53
KDM1A Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 reactome 65
KDM1A AndrogenReceptor netpath 167
KDM1A Coregulation of Androgen receptor activity pid 61
KDM1A Factors involved in megakaryocyte development and platelet production reactome 111
KDM1A HDACs deacetylate histones reactome 94
KDM1A HDMs demethylate histones reactome 49
KDM1A Notch-mediated HES/HEY network pid 48
KDM1A Notch signaling pathway pid 58
RUNX1 AndrogenReceptor netpath 167
RUNX1 Organic cation transport reactome 9
RUNX1 RANKL netpath 84
RUNX1 Regulation of nuclear SMAD2/3 signaling pid 82
RUNX1 TGF_beta_Receptor netpath 220
RUNX1 Validated transcriptional targets of deltaNp63 isoforms pid 47
RUNX1 Pathways in cancer kegg 321
RUNX1 Chronic myeloid leukemia kegg 69
RUNX1 Acute myeloid leukemia kegg 53
KDM1A Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 reactome 65
KDM1A AndrogenReceptor netpath 167
KDM1A Coregulation of Androgen receptor activity pid 61
KDM1A Factors involved in megakaryocyte development and platelet production reactome 111
KDM1A HDACs deacetylate histones reactome 94
KDM1A HDMs demethylate histones reactome 49
KDM1A Notch-mediated HES/HEY network pid 48
KDM1A Notch signaling pathway pid 58
RUNX1 AndrogenReceptor netpath 167
RUNX1 Organic cation transport reactome 9
RUNX1 RANKL netpath 84
RUNX1 Regulation of nuclear SMAD2/3 signaling pid 82
RUNX1 TGF_beta_Receptor netpath 220
RUNX1 Validated transcriptional targets of deltaNp63 isoforms pid 47
RUNX1 Pathways in cancer kegg 321
RUNX1 Chronic myeloid leukemia kegg 69
RUNX1 Acute myeloid leukemia kegg 53
KDM1A Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 reactome 65
KDM1A AndrogenReceptor netpath 167
KDM1A Coregulation of Androgen receptor activity pid 61
KDM1A Factors involved in megakaryocyte development and platelet production reactome 111
KDM1A HDACs deacetylate histones reactome 94
KDM1A HDMs demethylate histones reactome 49
KDM1A Notch-mediated HES/HEY network pid 48
KDM1A Notch signaling pathway pid 58
RUNX1 AndrogenReceptor netpath 167
RUNX1 Organic cation transport reactome 9
RUNX1 RANKL netpath 84
RUNX1 Regulation of nuclear SMAD2/3 signaling pid 82
RUNX1 TGF_beta_Receptor netpath 220
RUNX1 Validated transcriptional targets of deltaNp63 isoforms pid 47
RUNX1 Pathways in cancer kegg 321
RUNX1 Chronic myeloid leukemia kegg 69
RUNX1 Acute myeloid leukemia kegg 53
KDM1A Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 reactome 65
KDM1A AndrogenReceptor netpath 167
KDM1A Coregulation of Androgen receptor activity pid 61
KDM1A Factors involved in megakaryocyte development and platelet production reactome 111
KDM1A HDACs deacetylate histones reactome 94
KDM1A HDMs demethylate histones reactome 49
KDM1A Notch-mediated HES/HEY network pid 48
KDM1A Notch signaling pathway pid 58

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs