About Enhancer
| Enhancer ID: | E_02_0324 |
| Species: | human |
| Position : | chr6:31137269-31137697   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 29695788 |
| Enhancer experiment: | ATAC-seq,Luciferase Reporter Assay,ChIP-seq |
| Enhancer experiment description: | In human pre-implantation blastocyst, however, neither enhancer appears open, whereas two putative enhancers appear downstream of the POU5F1 TSS. Each of these peaks contains a cluster of AP2 sites and a KLF site, indicating that they could be opened by the combinatorial activity of these transcription factors during preimplantation development. |
About Target gene
| Target gene : | POU5F1(OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4),POU5F1(OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | -- |
| Target gene experiment description: | We found normal expression of OCT4 and self-renewal in the primed state, but a dramatic loss of OCT4 expression accompanied by differentiation upon reversion to the naive state. This indicates a potential direct role for TFAP2C in regulating the pluripotency master-regulator OCT4 by binding to a previously unknown enhancer, which in turn is likely to be important for preimplantation OCT4 expression.;We found normal expression of OCT4 and self-renewal in the primed state, but a dramatic loss of OCT4 expression accompanied by differentiation upon reversion to the naive state. This indicates a potential direct role for TFAP2C in regulating the pluripotency master-regulator OCT4 by binding to a previously unknown enhancer, which in turn is likely to be important for preimplantation OCT4 expression. |
About TF
| TF name : | TFAP2C(AP2-GAMMA,ERF1,TFAP2G,hAP-2g)TFAP2C(AP2-GAMMA,ERF1,TFAP2G,hAP-2g) |
| TF experiment: | Luciferase Reporter Assay |
| TF experiment description: | Luciferase activity from a pGL3 construct in which WT or mutant Intron Element 1 had been cloned, normalized to signal from a pGL3 construct with no enhancer. Results are shown from two independent experiment, except for the ?AP2 sample, for which there are n=3 replicates from two experiments. All signals were first normalized for Renilla signal.;Luciferase activity from a pGL3 construct in which WT or mutant Intron Element 1 had been cloned, normalized to signal from a pGL3 construct with no enhancer. Results are shown from two independent experiment, except for the ?AP2 sample, for which there are n=3 replicates from two experiments. All signals were first normalized for Renilla signal. |
About Function
| Enhancer function : | TFAP2C functions to maintain pluripotency and repress neuroectodermal differentiation during the transition from primed to naive by facilitating the opening of enhancers proximal to pluripotency factors. |
| Enhancer function experiment: | Luciferase Reporter Assay,CRISPR/Cas9 |
| Enhancer function experiment description: |
This indicates a potential direct role for TFAP2C in regulating the pluripotency master-regulator OCT4 by binding to a previously unknown Enhancer, which in turn is likely to be important for preimplantation OCT4 expression. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| TFAP2C | Validated transcriptional targets of TAp63 isoforms | pid | 54 |
| TFAP2C | Validated transcriptional targets of TAp63 isoforms | pid | 54 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene