About Enhancer
| Enhancer ID: | E_02_0340 |
| Species: | human |
| Position : | chr8:128206162-128314310   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | -- |
| Pubmed ID: | 29641996 |
| Enhancer experiment: | 4C,ChIP-seq |
| Enhancer experiment description: | Among the DNA loop structures observed in these datasets, a large 2.8 Mb DNA loop was evident in all four cell types. This loop connects CTCF sites encompassing the MYC gene and qualifies as an insulated neighborhood. The DNA anchor sites of this 2.8 Mb DNA loop occur at the boundaries of a TAD found in all cells. |
About Target gene
| Target gene : | MYC(MRTLC,bHLHe39,c-Myc),MYC(MRTLC,bHLHe39,c-Myc) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | ChIA-PET,ChIP-qPCR |
| Target gene experiment description: | CTCF chromatin immunoprecipitation quantative polymerase chain reaction (ChIP-qPCR) showed complete loss of CTCF binding to this site in K562 and HCT-116 cells and a 60%70% reduction in CTCF binding at this site in Jurkat and MCF7 cells. RNA analysis revealed a 70%80% reduction of endogenous MYC mRNA in the absence of the enhancer-docking site in all of these cell types.;CTCF chromatin immunoprecipitation quantative polymerase chain reaction (ChIP-qPCR) showed complete loss of CTCF binding to this site in K562 and HCT-116 cells and a 60%70% reduction in CTCF binding at this site in Jurkat and MCF7 cells. RNA analysis revealed a 70%80% reduction of endogenous MYC mRNA in the absence of the enhancer-docking site in all of these cell types. |
About TF
| TF name : | CTCF(MRD21)CTCF(MRD21) |
| TF experiment: | CRISPR/Cas9 |
| TF experiment description: | An inducible CRISPR/Cas9 perturbation model showed reduced proliferation for these four cell types upon induction of CTCF-site deletions .These results indicate that the CTCF motif in the MYC Enhancer-docking site is necessary for CTCF binding, for high levels of MYC expression and for cellular proliferation.;An inducible CRISPR/Cas9 perturbation model showed reduced proliferation for these four cell types upon induction of CTCF-site deletions .These results indicate that the CTCF motif in the MYC Enhancer-docking site is necessary for CTCF binding, for high levels of MYC expression and for cellular proliferation. |
About Function
| Enhancer function : | The CTCF site in the promoter-proximal region of MYC is important for optimal interaction with distal Enhancers and supports the idea that this CTCF site functions as an Enhancer-docking site. |
| Enhancer function experiment: | 4C,CRISPR/Cas9,ChIP-qPCR |
| Enhancer function experiment description: |
Similar results were obtained in HCT-116 cells, where the viewpoint was centered on the super-Enhancer located -0.4 Mb upstream of the MYC gene |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| CTCF | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
| CTCF | TGF_beta_Receptor | netpath | 220 |
| CTCF | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
| CTCF | TGF_beta_Receptor | netpath | 220 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene