About Enhancer
| Enhancer ID: | E_02_0389 |
| Species: | human |
| Position : | chr12:120299714-120301714   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Nothing |
| Pubmed ID: | 30442871 |
| Enhancer experiment: | qPCR,Immunofluorescence assays,Cell isolation, culture, and transfection,Site-directed mutagenesis,Knock down,EMSAs |
| Enhancer experiment description: | E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. |
About Target gene
| Target gene : | SIRT4,E2F1,HOXA5,IRF4,CREB1 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. ;E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. ;E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. ;E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. ;E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. |
About TF
| TF name : | -- |
| TF experiment: | qPCR,Immunofluorescence assays,Cell isolation, culture, and transfection,Site-directed mutagenesis,Knock down,EMSAs |
| TF experiment description: | E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. ;E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. ;E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. ;E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. ;E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. |
About Function
| Enhancer function : | E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
E2F1, CEBP?, and HOXA5 transcriptionally activate SIRT4 expression, whereas, IRF4, PAX4, and CREB1 transcriptionally repress SIRT4 expression. We further verified that SIRT4 knockdown could affect the ability of these transcription factors to regulate the differentiation of bovine adipocytes. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene