About Enhancer
| Enhancer ID: | E_02_0461 |
| Species: | human |
| Position : | chr20:30244800-30316534   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | Breast cancer |
| Pubmed ID: | 30139998 |
| Enhancer experiment: | ChIP-qPCR,ChIP-seq,Luciferase Reporter Assay |
| Enhancer experiment description: | In contrast to the broad RING1B ChIP-seq signals in pluripotent cells, RING1B peaks in the breast cell lines were narrow, resembling ChIP-seq signals of transcription factors. Therefore, we assessed whether RING1B is recruited to specific transcription factor-binding sites at SEs. |
About Target gene
| Target gene : | BCL2L1(BCL-XL/S,BCL2L,BCLX,Bcl-X,PPP1R52),BCL2L1(BCL-XL/S,BCL2L,BCLX,Bcl-X,PPP1R52),BCL2L1(BCL-XL/S,BCL2L,BCLX,Bcl-X,PPP1R52) |
| Strong evidence: | -- |
| Less strong evidence: | qRT-PCR |
| Target gene experiment description: | Indeed,we observed RING1B recruitment at SE regions near BCL2L1 in MDA-MB-231 and ESR1 in T47D23.;Indeed,we observed RING1B recruitment at SE regions near BCL2L1 in MDA-MB-231 and ESR1 in T47D23.;Indeed,we observed RING1B recruitment at SE regions near BCL2L1 in MDA-MB-231 and ESR1 in T47D23. |
About TF
| TF name : | RNF2(BAP-1,BAP1,DING,HIPI3,RING1B,RING2)RNF2(BAP-1,BAP1,DING,HIPI3,RING1B,RING2)RNF2(BAP-1,BAP1,DING,HIPI3,RING1B,RING2) |
| TF experiment: | ChIP-qPCR |
| TF experiment description: | RING1B, H3K27me3 and H3K27ac ChIP-qPCR of RIN1GB-containing enhancers in control and RING1B-depleted T47D cells. IgG antibody was used as a negative control. As additional control, RING1B ChIP-qPCR were performed using a different RING1B antibody from the one used for ChIP-seq.;RING1B, H3K27me3 and H3K27ac ChIP-qPCR of RIN1GB-containing enhancers in control and RING1B-depleted T47D cells. IgG antibody was used as a negative control. As additional control, RING1B ChIP-qPCR were performed using a different RING1B antibody from the one used for ChIP-seq.;RING1B, H3K27me3 and H3K27ac ChIP-qPCR of RIN1GB-containing enhancers in control and RING1B-depleted T47D cells. IgG antibody was used as a negative control. As additional control, RING1B ChIP-qPCR were performed using a different RING1B antibody from the one used for ChIP-seq. |
About Function
| Enhancer function : | RING1B regulates Enhancer activity and gene transcription not only by promoting the expression of oncogenes but also by regulating chromatin accessibility. |
| Enhancer function experiment: | ATAC-seq |
| Enhancer function experiment description: |
RING1B was recruited to regions targeted by transcription factors and its depletion deregulated breast cancer signaling pathways as well as FOXA1 and ER? localization to chromatin, we next hypothesized that RING1B regulates transcriptional programs in breast cancer by orchestrating chromatin accessibility. To test this, we performed transposase-accessible chromatin sequencing (ATAC-seq)35 in RING1B-depleted cells.These results indicate that RING1B depletion affects chromatin accessibility at enhancer regions. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| RNF2 | Oxidative Stress Induced Senescence | reactome | 120 |
| RNF2 | SUMOylation of DNA damage response and repair proteins | reactome | 76 |
| RNF2 | SUMOylation of RNA binding proteins | reactome | 46 |
| RNF2 | Oxidative Stress Induced Senescence | reactome | 120 |
| RNF2 | SUMOylation of DNA damage response and repair proteins | reactome | 76 |
| RNF2 | SUMOylation of RNA binding proteins | reactome | 46 |
| RNF2 | Oxidative Stress Induced Senescence | reactome | 120 |
| RNF2 | SUMOylation of DNA damage response and repair proteins | reactome | 76 |
| RNF2 | SUMOylation of RNA binding proteins | reactome | 46 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene