About Enhancer

Enhancer ID: E_02_0478
Species: human
Position : chr16:86422446-86426834
Biosample name:
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: --
Pubmed ID:  30119690
Enhancer experiment: ChIP-seq,Luciferase Reporter Assay,CRISPR/Cas9
Enhancer experiment description: Notably, two of these five sgRNAs,sgRNAs-AP169 and sgRNA-AP171, are independent sgRNAs that target the same enhancer region,hence increasing the confidence that these are true-positive hits. ENCODE ChIP-seq data confirmed a strong binding of both FOS and JUN to this region.

About Target gene

Target gene : FOXF1(ACDMPV,FKHL5,FREAC1)
Strong evidence: CRISPR/Cas9
Less strong evidence: qRT-PCR
Target gene experiment description: Furthermore,we identified FOXF1 as the tar_x0002_get gene of this Enhancer and demonstrated that it regu_x0002_lates the senescence phenotype.

About TF

TF name : JUN(AP-1,AP1,c-Jun,cJUN,p39)
TF experiment: CRISPR/Cas9,Luciferase Reporter Assay
TF experiment description: We thus constructed a CRISPR-Cas9 sgRNA library designed to target senescence-induced Enhancers that are putatively regulated by AP-1 and used it in a functional screen.That screen was confined to regions that are directly bound by p53 as detected by p53 chromatin immunoprecipitation-sequencing (ChIP-seq) analysis and therefore missed Enhancers that are critical for OIS enforcement and are regulated by other TFs, in either a p53-dependent or -independent manner.

About Function

Enhancer function : We identify FOXF1 as the gene regulated by this enhancer and demonstrate that FOXF1 mediates EnhAP1-OIS1 effect on the senescence phenotype.
Enhancer function experiment: qRT-PCR,Western blot
Enhancer function
experiment description:		 the physical interactions between EnhAP1-OIS1 and the promoter region of FOXF1 were significantly stronger , suggesting a robust transcriptional regulation of EnhAP1-OIS1.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
JUN Activation of anterior HOX genes in hindbrain development during early embryogenesis reactome 120
JUN Activation of the AP-1 family of transcription factors reactome 10
JUN Alpha6Beta4Integrin netpath 74
JUN AndrogenReceptor netpath 167
JUN Angiogenesis panther 141
JUN AP-1 transcription factor network pid 71
JUN Apoptosis signaling pathway panther 104
JUN ATF-2 transcription factor network pid 59
JUN B cell activation panther 52
JUN BCR netpath 161
JUN BCR signaling pathway pid 70
JUN Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
JUN Calcium signaling in the CD4+ TCR pathway pid 29
JUN CD40/CD40L signaling pid 36
JUN CDC42 signaling events pid 71
JUN Direct p53 effectors pid 141
JUN Downstream signaling in nave CD8+ T cells pid 69
JUN EGFR1 netpath 475
JUN Endothelins pid 64
JUN ErbB1 downstream signaling pid 105
JUN ErbB2/ErbB3 signaling events pid 41
JUN FAS signaling pathway panther 37
JUN Fc-epsilon receptor I signaling in mast cells pid 64
JUN FCERI mediated MAPK activation reactome 40
JUN FGF signaling pathway pid 55
JUN FOXA1 transcription factor network pid 45
JUN Gene expression of IL2 by AP-1 ( CD4 T cell receptor signaling (JNK cascade) ) inoh 5
JUN Gene expression of IL2 by AP-1 ( CD4 T cell receptor signaling ) inoh 5
JUN Glucocorticoid receptor regulatory network pid 85
JUN HIF-1-alpha transcription factor network pid 67
JUN Huntington disease panther 113
JUN IL1-mediated signaling events pid 34
JUN IL1 netpath 69
JUN IL12 signaling mediated by STAT4 pid 33
JUN IL2-mediated signaling events pid 54
JUN IL2 netpath 81
JUN IL5 netpath 59
JUN IL6-mediated signaling events pid 48
JUN IL6 netpath 85
JUN Inflammation mediated by chemokine and cytokine signaling pathway panther 189
JUN Integrin-linked kinase signaling pid 46
JUN Intracellular Signalling Through Adenosine Receptor A2a and Adenosine smpdb 37
JUN Intracellular Signalling Through Adenosine Receptor A2b and Adenosine smpdb 38
JUN JNK cascade ( CD4 T cell receptor signaling (JNK cascade) ) inoh 12
JUN JNK cascade ( CD4 T cell receptor signaling ) inoh 10
JUN JNK cascade ( TGF-beta signaling(through TAK1) ) inoh 6
JUN JNK cascade ( Toll-like receptor signaling pathway (through ECSIT, MEKK1, MKKs, JNK cascade) ) inoh 12
JUN JNK signaling in the CD4+ TCR pathway pid 14
JUN KitReceptor netpath 104
JUN LPA receptor mediated events pid 65
JUN MAPK6/MAPK4 signaling reactome 90
JUN Nephrin/Neph1 signaling in the kidney podocyte pid 28
JUN Osteopontin-mediated events pid 32
JUN Oxidative Stress Induced Senescence reactome 120
JUN Oxidative stress response panther 23
JUN PDGF signaling pathway panther 113
JUN PDGFR-alpha signaling pathway pid 22
JUN PDGFR-beta signaling pathway pid 125
JUN Pre-NOTCH Transcription and Translation reactome 29
JUN Presenilin action in Notch and Wnt signaling pid 46
JUN Prolactin netpath 105
JUN RAC1 signaling pathway pid 54
JUN RANKL netpath 84
JUN Ras Pathway panther 69
JUN Regulation of Androgen receptor activity pid 54
JUN Regulation of nuclear beta catenin signaling and target gene transcription pid 80
JUN Regulation of nuclear SMAD2/3 signaling pid 82
JUN Regulation of retinoblastoma protein pid 67
JUN Regulation of Telomerase pid 70
JUN RhoA signaling pathway pid 46
JUN S1P2 pathway pid 26
JUN Senescence-Associated Secretory Phenotype (SASP) reactome 108
JUN Signaling events mediated by focal adhesion kinase pid 63
JUN Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met) pid 80
JUN Signaling events regulated by Ret tyrosine kinase pid 39
JUN Signaling mediated by p38-alpha and p38-beta pid 35
JUN T cell activation panther 60
JUN TCR netpath 261
JUN TGF-beta signaling pathway panther 88
JUN TGF_beta_Receptor netpath 220
JUN TNFalpha netpath 274
JUN Toll receptor signaling pathway panther 44
JUN TP53 Regulates Transcription of DNA Repair Genes reactome 61
JUN Validated nuclear estrogen receptor alpha network pid 65
JUN Validated transcriptional targets of AP1 family members Fra1 and Fra2 pid 37
JUN Wnt netpath 118
JUN JNK cascade ( IL-1 signaling pathway (through JNK cascade) ) inoh 16
JUN JNK cascade ( Toll-like receptor signaling pathway (through JNK cascade) ) inoh 12
JUN MAPK signaling pathway kegg 264
JUN ErbB signaling pathway kegg 83
JUN Wnt signaling pathway kegg 135
JUN Focal adhesion kegg 197
JUN Toll-like receptor signaling pathway kegg 100
JUN T cell receptor signaling pathway kegg 105
JUN B cell receptor signaling pathway kegg 70
JUN Neurotrophin signaling pathway kegg 126
JUN GnRH signaling pathway kegg 98
JUN Epithelial cell signaling in Helicobacter pylori infection kegg 67
JUN Leishmaniasis kegg 71
JUN Chagas disease kegg 103
JUN Pathways in cancer kegg 321
JUN Colorectal cancer kegg 63
JUN Renal cell carcinoma kegg 65
JUN Hs_MAPK_Signaling_Pathway_WP382_79951 wikipathways 58
JUN Hs_Hepatitis_C_and_Hepatocellular_Carcinoma_WP3646_88640 wikipathways 36
JUN Hs_Apoptosis_WP254_88977 wikipathways 31
JUN Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
JUN Hs_T-Cell_antigen_Receptor_(TCR)_pathway_during_Staphylococcus_aureus_infection_WP3863_90702 wikipathways 35
JUN Hs_Apoptosis_Modulation_and_Signaling_WP1772_91293 wikipathways 71
JUN Hs_PDGF_Pathway_WP2526_82681 wikipathways 12
JUN Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 wikipathways 153
JUN Hs_ATM_Signaling_Pathway_WP2516_90247 wikipathways 32
JUN Hs_BMP_Signaling_Pathway_in_Eyelid_Development_WP3927_90735 wikipathways 11
JUN Hs_Nucleotide_Metabolism_WP404_68960 wikipathways 16
JUN Hs_TNF_alpha_Signaling_Pathway_WP231_89895 wikipathways 61
JUN Hs_Estrogen_signaling_pathway_WP712_78491 wikipathways 13
JUN Hs_IL-5_Signaling_Pathway_WP127_78498 wikipathways 35
JUN Hs_TGF-beta_Signaling_Pathway_WP366_90028 wikipathways 67
JUN Hs_Brain-Derived_Neurotrophic_Factor_(BDNF)_signaling_pathway_WP2380_89803 wikipathways 80
JUN Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 wikipathways 107
JUN Hs_T-Cell_antigen_Receptor_(TCR)_Signaling_Pathway_WP69_89937 wikipathways 60
JUN Hs_IL-1_signaling_pathway_WP195_89932 wikipathways 34
JUN Hs_IL-3_Signaling_Pathway_WP286_78583 wikipathways 35
JUN Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900 wikipathways 71

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs