About Enhancer
| Enhancer ID: | E_02_0489 |
| Species: | human |
| Position : | chr14:100235593-100237593   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 30098214 |
| Enhancer experiment: | RT ? PCR, Weston blot, flow cytometry, statistical analysis, chromatin immunoprecipitation assays, gene knockdown |
| Enhancer experiment description: | Ei and E3 are both required for Ig? gene rearrangement during early stages of B cell development, whereas optimal expression of the rearranged Ig? gene relies on both E3 and Ed. The transcription factor YY1 affects the expression of many genes involved in B cell development, probably by mediating interactions between their enhancers and promoters.Herein, we found that YY1 binds to the E3 enhancer and suppresses Ig? expression in B lymphoma cells by epigenetically modifying the enhancer. Knocking down YY1 enhanced Ig? expression, which was associated with increased levels of E2A (encoded by the TCF3 gene) and its binding to the E3 enhancer. |
About Target gene
| Target gene : | YY1(DELTA,GADEVS,INO80S,NF-E1,UCRBP,YIN-YANG-1),TCF3 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Ei and E3 are both required for Ig? gene rearrangement during early stages of B cell development, whereas optimal expression of the rearranged Ig? gene relies on both E3 and Ed. The transcription factor YY1 affects the expression of many genes involved in B cell development, probably by mediating interactions between their enhancers and promoters.Herein, we found that YY1 binds to the E3 enhancer and suppresses Ig? expression in B lymphoma cells by epigenetically modifying the enhancer. Knocking down YY1 enhanced Ig? expression, which was associated with increased levels of E2A (encoded by the TCF3 gene) and its binding to the E3 enhancer. ;Ei and E3 are both required for Ig? gene rearrangement during early stages of B cell development, whereas optimal expression of the rearranged Ig? gene relies on both E3 and Ed. The transcription factor YY1 affects the expression of many genes involved in B cell development, probably by mediating interactions between their enhancers and promoters.Herein, we found that YY1 binds to the E3 enhancer and suppresses Ig? expression in B lymphoma cells by epigenetically modifying the enhancer. Knocking down YY1 enhanced Ig? expression, which was associated with increased levels of E2A (encoded by the TCF3 gene) and its binding to the E3 enhancer. |
About TF
| TF name : | -- |
| TF experiment: | RT?PCR,weston blot,?????,????,Chromatin immunoprecipitation assays,???? |
| TF experiment description: | Ei and E3 are both required for Ig? gene rearrangement during early stages of B cell development, whereas optimal expression of the rearranged Ig? gene relies on both E3 and Ed. The transcription factor YY1 affects the expression of many genes involved in B cell development, probably by mediating interactions between their enhancers and promoters.Herein, we found that YY1 binds to the E3 enhancer and suppresses Ig? expression in B lymphoma cells by epigenetically modifying the enhancer. Knocking down YY1 enhanced Ig? expression, which was associated with increased levels of E2A (encoded by the TCF3 gene) and its binding to the E3 enhancer. ;Ei and E3 are both required for Ig? gene rearrangement during early stages of B cell development, whereas optimal expression of the rearranged Ig? gene relies on both E3 and Ed. The transcription factor YY1 affects the expression of many genes involved in B cell development, probably by mediating interactions between their enhancers and promoters.Herein, we found that YY1 binds to the E3 enhancer and suppresses Ig? expression in B lymphoma cells by epigenetically modifying the enhancer. Knocking down YY1 enhanced Ig? expression, which was associated with increased levels of E2A (encoded by the TCF3 gene) and its binding to the E3 enhancer. |
About Function
| Enhancer function : | Ei and E3 are both required for Ig? gene rearrangement during early stages of B cell development, whereas optimal expression of the rearranged Ig? gene relies on both E3 and Ed. The transcription factor YY1 affects the expression of many genes involved in B cell development, probably by mediating interactions between their enhancers and promoters.Herein, we found that YY1 binds to the E3 enhancer and suppresses Ig? expression in B lymphoma cells by epigenetically modifying the enhancer. Knocking down YY1 enhanced Ig? expression, which was associated with increased levels of E2A (encoded by the TCF3 gene) and its binding to the E3 enhancer. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Ei and E3 are both required for Ig? gene rearrangement during early stages of B cell development, whereas optimal expression of the rearranged Ig? gene relies on both E3 and Ed. The transcription factor YY1 affects the expression of many genes involved in B cell development, probably by mediating interactions between their enhancers and promoters.Herein, we found that YY1 binds to the E3 enhancer and suppresses Ig? expression in B lymphoma cells by epigenetically modifying the enhancer. Knocking down YY1 enhanced Ig? expression, which was associated with increased levels of E2A (encoded by the TCF3 gene) and its binding to the E3 enhancer. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene