About Enhancer
| Enhancer ID: | E_02_0516 |
| Species: | human |
| Position : | chr8:38291071-38304326   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | -- |
| Pubmed ID: | 30033119 |
| Enhancer experiment: | ChIP-STARR-seq |
| Enhancer experiment description: | Visual inspection of selected genomic regions illustrates the broad spectrum of enhancer activity measured by ChIP-STARR-seq. For instance, ChIP-seq for NANOG indicates two strong binding sites up- and downstream of SOX2, but only the downstream binding site resulted in ChIP-STARR-seq RNA in excess of plasmid abundance. |
About Target gene
| Target gene : | FGFR1(BFGFR,CD331,CEK,ECCL,FGFBR,FGFR-1,FLG,FLT-2,FLT2,HBGFR,HH2,HRTFDS,KAL2,N-SAM,OGD,bFGF-R-1),FGFR1(BFGFR,CD331,CEK,ECCL,FGFBR,FGFR-1,FLG,FLT-2,FLT2,HBGFR,HH2,HRTFDS,KAL2,N-SAM,OGD,bFGF-R-1) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | Luciferase Reporter Assay,qRT-PCR |
| Target gene experiment description: | Luciferase assays confirmed spatially restricted Enhancer activity of DNA in the neighborhood of the central active region of the FGFR1 SE.;Luciferase assays confirmed spatially restricted Enhancer activity of DNA in the neighborhood of the central active region of the FGFR1 SE. |
About TF
| TF name : | POU5F1 (OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4)POU5F1 (OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4) |
| TF experiment: | ChIP-qPCR |
| TF experiment description: | To investigate the functional potential of enhancers in ESCs, we first focused on primed H9 ESCs (Figures S1A and S1B) and performed ChIP for NANOG, OCT4, H3K4me1 and H3K27ac.ChIP-qPCR and ChIP-seq were similar to previous results (Figures S1C and 1D).Applying this threshold to regions bound by NANOG, OCT4, H3K4me1, H3K27ac, or combinations of these factors indicates that only a minority of ChIP-seq peaks showed enhancer activity (Figure 2D and S3C), with regions bound by OCT4 having the highest proportion of high activity enhancers.;To investigate the functional potential of enhancers in ESCs, we first focused on primed H9 ESCs (Figures S1A and S1B) and performed ChIP for NANOG, OCT4, H3K4me1 and H3K27ac.ChIP-qPCR and ChIP-seq were similar to previous results (Figures S1C and 1D).Applying this threshold to regions bound by NANOG, OCT4, H3K4me1, H3K27ac, or combinations of these factors indicates that only a minority of ChIP-seq peaks showed enhancer activity (Figure 2D and S3C), with regions bound by OCT4 having the highest proportion of high activity enhancers. |
About Function
| Enhancer function : | Although transposable elements associate with putative enhancers, only some exhibit activity. |
| Enhancer function experiment: | Luciferase Reporter Assay,CRISPR/Cas9 |
| Enhancer function experiment description: |
Luciferase assays confirmed spatially restricted Enhancer activity of DNA in the neighborhood of the central active region of the FGFR1 SE |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene