About Enhancer
| Enhancer ID: | E_02_0518 |
| Species: | human |
| Position : | chr10:88950934-88952934   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 30031040 |
| Enhancer experiment: | Q-PCR, MTT assay, immunoblotting, Western blot |
| Enhancer experiment description: | PFBS treatments significantly increased the protein and mRNA levels of the master transcription factors in adipocyte differentiation; CCAAT/enhancer-binding protein ? (C/EBP?) and peroxisome proliferator-activated receptor gamma (PPAR?), along with acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), the key proteins in lipogenesis. Previously, PFASs, particularly PFOS and perfluorooctanoic acid (PFOA), were reported to promote adipogenesis in 3T3-L1 adipocytes with increased expression of adipogenic genes, including CCAAT/enhancer-binding protein alpha (C/EBP?), peroxisome proliferator-activated receptor gamma (PPAR?), fatty acid binding protein 4 (FABP4) and lipoprotein lipase (LPL) (Watkins et al. 2015, Yamamoto et al. |
About Target gene
| Target gene : | FAS,FABP4 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | PFBS treatments significantly increased the protein and mRNA levels of the master transcription factors in adipocyte differentiation; CCAAT/enhancer-binding protein ? (C/EBP?) and peroxisome proliferator-activated receptor gamma (PPAR?), along with acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), the key proteins in lipogenesis. Previously, PFASs, particularly PFOS and perfluorooctanoic acid (PFOA), were reported to promote adipogenesis in 3T3-L1 adipocytes with increased expression of adipogenic genes, including CCAAT/enhancer-binding protein alpha (C/EBP?), peroxisome proliferator-activated receptor gamma (PPAR?), fatty acid binding protein 4 (FABP4) and lipoprotein lipase (LPL) (Watkins et al. 2015, Yamamoto et al.;PFBS treatments significantly increased the protein and mRNA levels of the master transcription factors in adipocyte differentiation; CCAAT/enhancer-binding protein ? (C/EBP?) and peroxisome proliferator-activated receptor gamma (PPAR?), along with acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), the key proteins in lipogenesis. Previously, PFASs, particularly PFOS and perfluorooctanoic acid (PFOA), were reported to promote adipogenesis in 3T3-L1 adipocytes with increased expression of adipogenic genes, including CCAAT/enhancer-binding protein alpha (C/EBP?), peroxisome proliferator-activated receptor gamma (PPAR?), fatty acid binding protein 4 (FABP4) and lipoprotein lipase (LPL) (Watkins et al. 2015, Yamamoto et al. |
About TF
| TF name : | -- |
| TF experiment: | q-PCR,MTT?,Immunoblotting,western blot |
| TF experiment description: | PFBS treatments significantly increased the protein and mRNA levels of the master transcription factors in adipocyte differentiation; CCAAT/enhancer-binding protein ? (C/EBP?) and peroxisome proliferator-activated receptor gamma (PPAR?), along with acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), the key proteins in lipogenesis. Previously, PFASs, particularly PFOS and perfluorooctanoic acid (PFOA), were reported to promote adipogenesis in 3T3-L1 adipocytes with increased expression of adipogenic genes, including CCAAT/enhancer-binding protein alpha (C/EBP?), peroxisome proliferator-activated receptor gamma (PPAR?), fatty acid binding protein 4 (FABP4) and lipoprotein lipase (LPL) (Watkins et al. 2015, Yamamoto et al.;PFBS treatments significantly increased the protein and mRNA levels of the master transcription factors in adipocyte differentiation; CCAAT/enhancer-binding protein ? (C/EBP?) and peroxisome proliferator-activated receptor gamma (PPAR?), along with acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), the key proteins in lipogenesis. Previously, PFASs, particularly PFOS and perfluorooctanoic acid (PFOA), were reported to promote adipogenesis in 3T3-L1 adipocytes with increased expression of adipogenic genes, including CCAAT/enhancer-binding protein alpha (C/EBP?), peroxisome proliferator-activated receptor gamma (PPAR?), fatty acid binding protein 4 (FABP4) and lipoprotein lipase (LPL) (Watkins et al. 2015, Yamamoto et al. |
About Function
| Enhancer function : | PFBS treatments significantly increased the protein and mRNA levels of the master transcription factors in adipocyte differentiation; CCAAT/enhancer-binding protein ? (C/EBP?) and peroxisome proliferator-activated receptor gamma (PPAR?), along with acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), the key proteins in lipogenesis. Previously, PFASs, particularly PFOS and perfluorooctanoic acid (PFOA), were reported to promote adipogenesis in 3T3-L1 adipocytes with increased expression of adipogenic genes, including CCAAT/enhancer-binding protein alpha (C/EBP?), peroxisome proliferator-activated receptor gamma (PPAR?), fatty acid binding protein 4 (FABP4) and lipoprotein lipase (LPL) (Watkins et al. 2015, Yamamoto et al. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
PFBS treatments significantly increased the protein and mRNA levels of the master transcription factors in adipocyte differentiation; CCAAT/enhancer-binding protein ? (C/EBP?) and peroxisome proliferator-activated receptor gamma (PPAR?), along with acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), the key proteins in lipogenesis. Previously, PFASs, particularly PFOS and perfluorooctanoic acid (PFOA), were reported to promote adipogenesis in 3T3-L1 adipocytes with increased expression of adipogenic genes, including CCAAT/enhancer-binding protein alpha (C/EBP?), peroxisome proliferator-activated receptor gamma (PPAR?), fatty acid binding protein 4 (FABP4) and lipoprotein lipase (LPL) (Watkins et al. 2015, Yamamoto et al. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene