About Enhancer
| Enhancer ID: | E_02_0521 |
| Species: | mouse |
| Position : | chr9:32544664-32546664   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Inflammation |
| Pubmed ID: | 30026272 |
| Enhancer experiment: | QRT PCR, immunofluorescence staining, statistical analysis |
| Enhancer experiment description: | Approach and Results We screened various combinations of transcription factors, including Myocd, Mef2C, Mef2B, Mkl1, Gata4, Gata5, Gata6, Ets1, and their corresponding carboxyterminal fusions to the transactivation domain of MyoD indicated by *-, for their effects on reprogramming mouse embryonic fibroblasts (MEFs) and human adult dermal fibroblasts (hFBs) to the smooth muscle cell fate as determined by the expression of specific markers. |
About Target gene
| Target gene : | Ets1,Gata4(Gata-4) |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Approach and Results We screened various combinations of transcription factors, including Myocd, Mef2C, Mef2B, Mkl1, Gata4, Gata5, Gata6, Ets1, and their corresponding carboxyterminal fusions to the transactivation domain of MyoD indicated by *-, for their effects on reprogramming mouse embryonic fibroblasts (MEFs) and human adult dermal fibroblasts (hFBs) to the smooth muscle cell fate as determined by the expression of specific markers.;Approach and Results We screened various combinations of transcription factors, including Myocd, Mef2C, Mef2B, Mkl1, Gata4, Gata5, Gata6, Ets1, and their corresponding carboxyterminal fusions to the transactivation domain of MyoD indicated by *-, for their effects on reprogramming mouse embryonic fibroblasts (MEFs) and human adult dermal fibroblasts (hFBs) to the smooth muscle cell fate as determined by the expression of specific markers. |
About TF
| TF name : | -- |
| TF experiment: | qRT-PCR,??????,???? |
| TF experiment description: | Approach and Results We screened various combinations of transcription factors, including Myocd, Mef2C, Mef2B, Mkl1, Gata4, Gata5, Gata6, Ets1, and their corresponding carboxyterminal fusions to the transactivation domain of MyoD indicated by *-, for their effects on reprogramming mouse embryonic fibroblasts (MEFs) and human adult dermal fibroblasts (hFBs) to the smooth muscle cell fate as determined by the expression of specific markers.;Approach and Results We screened various combinations of transcription factors, including Myocd, Mef2C, Mef2B, Mkl1, Gata4, Gata5, Gata6, Ets1, and their corresponding carboxyterminal fusions to the transactivation domain of MyoD indicated by *-, for their effects on reprogramming mouse embryonic fibroblasts (MEFs) and human adult dermal fibroblasts (hFBs) to the smooth muscle cell fate as determined by the expression of specific markers. |
About Function
| Enhancer function : | Approach and Results We screened various combinations of transcription factors, including Myocd, Mef2C, Mef2B, Mkl1, Gata4, Gata5, Gata6, Ets1, and their corresponding carboxyterminal fusions to the transactivation domain of MyoD indicated by *-, for their effects on reprogramming mouse embryonic fibroblasts (MEFs) and human adult dermal fibroblasts (hFBs) to the smooth muscle cell fate as determined by the expression of specific markers. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Approach and Results We screened various combinations of transcription factors, including Myocd, Mef2C, Mef2B, Mkl1, Gata4, Gata5, Gata6, Ets1, and their corresponding carboxyterminal fusions to the transactivation domain of MyoD indicated by *-, for their effects on reprogramming mouse embryonic fibroblasts (MEFs) and human adult dermal fibroblasts (hFBs) to the smooth muscle cell fate as determined by the expression of specific markers. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene