About Enhancer
| Enhancer ID: |
E_02_0549 |
| Species: |
human |
| Position :
|
chr4:108044755-108046755
  |
| Biosample name: |
|
| Experiment class :
|
High+Lowthroughput |
| Enhancer type: |
Enhancer |
| Disease: |
Hepatocellular carcinoma (hcc) |
| Pubmed ID: |
29953980
|
| Enhancer experiment: |
Real time quantitative PCR, chromatin immunoprecipitation (chip) assay, statistical analysis, immunofluorescence staining |
| Enhancer experiment description: |
Overexpression of LEF1 diminished repression of Wnt signalling and reduced proliferation activity in mangiferin-treated HCC cells. The mangiferin-mediated down-regulation of LEF1 was independent of ?-catenin but associated with WT1 protein. |
About Target gene
| Target gene : |
LEF1,WT1(AWT1,GUD,NPHS4,WAGR,WIT-2,WT33) |
| Strong evidence: |
qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: |
RNA-Seq |
| Target gene experiment description: |
Overexpression of LEF1 diminished repression of Wnt signalling and reduced proliferation activity in mangiferin-treated HCC cells. The mangiferin-mediated down-regulation of LEF1 was independent of ?-catenin but associated with WT1 protein.;Overexpression of LEF1 diminished repression of Wnt signalling and reduced proliferation activity in mangiferin-treated HCC cells. The mangiferin-mediated down-regulation of LEF1 was independent of ?-catenin but associated with WT1 protein. |
About TF
| TF name : |
-- |
| TF experiment: |
Real-time Quantitative PCR,Chromatin immunoprecipitation (ChIP) assay,????,?????? |
| TF experiment description: |
Overexpression of LEF1 diminished repression of Wnt signalling and reduced proliferation activity in mangiferin-treated HCC cells. The mangiferin-mediated down-regulation of LEF1 was independent of ?-catenin but associated with WT1 protein.;Overexpression of LEF1 diminished repression of Wnt signalling and reduced proliferation activity in mangiferin-treated HCC cells. The mangiferin-mediated down-regulation of LEF1 was independent of ?-catenin but associated with WT1 protein. |
About Function
| Enhancer function : |
Overexpression of LEF1 diminished repression of Wnt signalling and reduced proliferation activity in mangiferin-treated HCC cells. The mangiferin-mediated down-regulation of LEF1 was independent of ?-catenin but associated with WT1 protein. |
| Enhancer function experiment: |
Immunohistochemical staining |
Enhancer function
experiment description: |
Overexpression of LEF1 diminished repression of Wnt signalling and reduced proliferation activity in mangiferin-treated HCC cells. The mangiferin-mediated down-regulation of LEF1 was independent of ?-catenin but associated with WT1 protein. |
About SNP
Upstream Pathway Annotation of TF
| GeneName |
Pathway Name |
Source |
Gene Number |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene
Expression of target genes for the
enhancer
Enhancer associated SNPs