About Enhancer
| Enhancer ID: | E_02_0573 |
| Species: | human |
| Position : | chr3:41191825-41193825   |
| Biosample name: | |
| Experiment class : | High+Lowthroughput |
| Enhancer type: | Enhancer |
| Disease: | Primary aldosteronism |
| Pubmed ID: | 33287648 |
| Enhancer experiment: | CTNNB1 Knockdown?Total RNA Extraction and qRT-PCR?Western Blot Analysis?Cell Viability Assays?Quantification of Aldosterone Using ELISA Kit |
| Enhancer experiment description: | Previous studies have suggested that there are somatic CTNNB1 mutations in APA, but the specific mechanism of CTNNB1 mutation in APA tumorigenesis and aldosterone secretion remains unclear. In the present study, human adrenocortical carcinoma cell line H295 R was used to establish stable CTNNB1 knockdown cell lines. |
About Target gene
| Target gene : | CTNNB1 |
| Strong evidence: | qRT-PCR,qPCR,ChIP,3C |
| Less strong evidence: | RNA-Seq |
| Target gene experiment description: | Previous studies have suggested that there are somatic CTNNB1 mutations in APA, but the specific mechanism of CTNNB1 mutation in APA tumorigenesis and aldosterone secretion remains unclear. In the present study, human adrenocortical carcinoma cell line H295 R was used to establish stable CTNNB1 knockdown cell lines. |
About TF
| TF name : | -- |
| TF experiment: | CTNNB1 Knockdown?Total RNA Extraction and qRT-PCR?Western Blot Analysis?Cell Viability Assays?Quantification of Aldosterone Using ELISA Kit |
| TF experiment description: | Previous studies have suggested that there are somatic CTNNB1 mutations in APA, but the specific mechanism of CTNNB1 mutation in APA tumorigenesis and aldosterone secretion remains unclear. In the present study, human adrenocortical carcinoma cell line H295 R was used to establish stable CTNNB1 knockdown cell lines. |
About Function
| Enhancer function : | Previous studies have suggested that there are somatic CTNNB1 mutations in APA, but the specific mechanism of CTNNB1 mutation in APA tumorigenesis and aldosterone secretion remains unclear. In the present study, human adrenocortical carcinoma cell line H295 R was used to establish stable CTNNB1 knockdown cell lines. |
| Enhancer function experiment: | Immunohistochemical staining |
| Enhancer function experiment description: |
Previous studies have suggested that there are somatic CTNNB1 mutations in APA, but the specific mechanism of CTNNB1 mutation in APA tumorigenesis and aldosterone secretion remains unclear. In the present study, human adrenocortical carcinoma cell line H295 R was used to establish stable CTNNB1 knockdown cell lines. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene