About Enhancer
| Enhancer ID: | E_02_0747 |
| Species: | human |
| Position : | chr6:31139519-31140167   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 30590035 |
| Enhancer experiment: | ATAC-seq,ChIP-seq |
| Enhancer experiment description: | These data show that the PE, DE, and NE are all open in hPGCLCs at day 4 of aggregate differentiation, with the NE being more open in hPGCs than the DE and PE. This observation raises the possibility that restriction of OCT4 expression between days 2 and 3 of aggregate differentiation may be due to NE and/or the DE enhancer activation at the OCT4 locus. Given that the NE is bound by TFAP2C in groundstate naive pluripotent stem cells, whereas the DE is not , we next confirmed that the OCT4 NE is also a target of TFAP2C during hPGCLC differentiation. |
About Target gene
| Target gene : | POU5F1(OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-qPCR |
| Target gene experiment description: | Screenshot of ATAC-seq and TFAP2C ChIP-seq signals showing three Enhancers at the POU5F1 locus (encoding OCT4). Shaded boxes highlight the naive Enhancer(NE),proximalEnhancer(PE),anddistalEnhancer(DE)atthePOU5F1locus.DEdeletion andNEdeletion indicategenomicregionsthatweredeleted by CRISPR/Cas9-mediated genome editing. Primers for ChIP-qPCR (P1-2,P3-4,P5-6) of NE and control regions are shown. |
About TF
| TF name : | TFAP2C(AP2-GAMMA,ERF1,TFAP2G,hAP-2g) |
| TF experiment: | CRISPR/Cas9 |
| TF experiment description: | Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive Enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity. |
About Function
| Enhancer function : | TFAP2C regulates human germline cell formation is opening naive-specific Enhancers, with one of these Enhancers corresponding to the NE at the OCT4 locus. |
| Enhancer function experiment: | CRISPR/Cas9 |
| Enhancer function experiment description: |
Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| TFAP2C | Validated transcriptional targets of TAp63 isoforms | pid | 54 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene