About Enhancer
| Enhancer ID: | E_02_0750 |
| Species: | mouse |
| Position : | chr10:50623698-50625698   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Haploinsufficiency |
| Pubmed ID: | 30545847 |
| Enhancer experiment: | ChIP-seq,ATAC-seq |
| Enhancer experiment description: | CRISPRa targeting of the Sim1 promoter or its hypothalamusspecific enhancer, which is 270 kilobases away from the gene, in Sim1 haploinsufficient mice increased the expression of the normal copy of Sim1. |
About Target gene
| Target gene : | Sim1(bHLHe14,mSIM1),Sim1(bHLHe14,mSIM1) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq,PCR,Transgenic mice |
| Target gene experiment description: | We also carried out ChIP-seq using an antibody against S. pyogenes Cas9 in both CRISPRa-promoter and CRISPRa-Enhancer transfected cells and found on-target binding for the promoter and Enhancer,respectively.;We also carried out ChIP-seq using an antibody against S. pyogenes Cas9 in both CRISPRa-promoter and CRISPRa-Enhancer transfected cells and found on-target binding for the promoter and Enhancer,respectively. |
About TF
| TF name : | Sim1(bHLHe14,mSIM1)Sim1(bHLHe14,mSIM1) |
| TF experiment: | CRISPR/Cas9,qPCR |
| TF experiment description: | CRISPRa targeting of the Sim1 promoter or its hypothalamusspecific enhancer, which is 270 kilobases away from the gene, in Sim1 haploinsufficient mice increased the expression of the normal copy of Sim1. This up-regulation was sufficient to rescue the obesity phenotype of Sim1 heterozygous mice and led to significantly reduced food intake and body fat content in thesemice.;CRISPRa targeting of the Sim1 promoter or its hypothalamusspecific enhancer, which is 270 kilobases away from the gene, in Sim1 haploinsufficient mice increased the expression of the normal copy of Sim1. This up-regulation was sufficient to rescue the obesity phenotype of Sim1 heterozygous mice and led to significantly reduced food intake and body fat content in thesemice. |
About Function
| Enhancer function : | Transgenic-based CRISPRa targeting of the Sim1 promoter or its distant hypothalamic enhancer up-regulated its expression from the endogenous functional allele in a tissue-specific manner, rescuing the obesity phenotype in Sim1 heterozygous mice. |
| Enhancer function experiment: | CRISPR/Cas9,ChIP-seq |
| Enhancer function experiment description: |
Transgenic-based CRISPRa targeting of the Sim1 promoter or its distant hypothalamic enhancer up-regulated its expression from the endogenous functional allele in a tissue-specific manner, rescuing the obesity phenotype in Sim1 heterozygous mice. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene