About Enhancer
| Enhancer ID: | E_02_0751 |
| Species: | human |
| Position : | chr17:30249928-30278499   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 30527662 |
| Enhancer experiment: | ChIP-seq,ChIA-PET |
| Enhancer experiment description: | The data show that the promoters are located within Smc1 ChIA-PET loops,the anchors of which align with strong cohesin and CTCF ChIP-seq peaks lacking H3K27ac ( These insulated neighborhoods encompass the TSS(arrow),promoters (vertical gray bars),and potential Enhancers of Mitf and Sik1 (vertical green bar). View of a genomic region around Mitf integrating Smc1 ChIA-PET,and Smc1,Smc3,Rad21,CTCF,Med1,and H3K27ac ChIP-seq with 4C data and promoter capture Hi-C data. |
About Target gene
| Target gene : | Sik1(Hrt-20,Msk,Sik,Sik-1,Snf1lk) |
| Strong evidence: | 4C |
| Less strong evidence: | Hi-C,ChIP-seq,ChIA-PET |
| Target gene experiment description: | High-resolution 4C was employed using promoter viewpoints to determine whether individual genes fit into the framework described in Figure 1.Figures 2A and 2B compare the Mitf and Sik1 genes expressed at low(RPKM = 2.2) and modest (RPKM = 21) levels, respectively, as measured by mRNA-seq and nascent RNA-seq (RPKM 0.53 and 4.2 for Mitf and Sik1). The data show that the promoters are located within Smc1 ChIA-PET loops, the anchors of which align with strong cohesin and CTCF ChIP-seq peaks (vertical red bars) lacking H3K27ac. |
About TF
| TF name : | Esrrb(Err2,Errb,Estrrb,Nr3b2)Med1(AI480703,CRSP210,DRIP205,PBP,Pparbp,TRAP220,TRIP-2,l11Jus15) |
| TF experiment: | ChIP,Immobilized Template Assay,siRNA,Western blot |
| TF experiment description: | Consistent with the biochemical data, knockdown of Esrrb by small interfering RNA (siRNA) (Esrrb KD) in cells causes diminished recruitment of Mediator (Med1 and Med12) to Esrrb binding sites genome-wide.For example, upon Esrrb KD, Med1 and Med12 binding decreases at Esrrb sites within the enhancer (Enh) of the divergently transcribed Slc13a5 and Xaf1 genes.In sum, on select genes, Esrrb KD can be used to deplete the Mediator and inactivate Enhancers, either partially or near fully, to downregulate transcription of their target genes. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene