About Enhancer
| Enhancer ID: |
E_02_0754 |
| Species: |
human |
| Position :
|
chr2:234665349-234665739
  |
| Biosample name: |
|
| Experiment class :
|
Low throughput |
| Enhancer type: |
Enhancer |
| Disease: |
Hepatocellular carcinoma |
| Pubmed ID: |
30397001
|
| Enhancer experiment: |
Luciferase Reporter Assay,qRT-PCR |
| Enhancer experiment description: |
These observations suggested that a specific enhancer region, such as neighboring transcriptional factors, is required for CAR/CCAR1 interaction. |
About Target gene
| Target gene : |
UGT1A1(BILIQTL1,GNT1,HUG-BR1,UDPGT,UDPGT1-1,UGT1,UGT1A) |
| Strong evidence: |
-- |
| Less strong evidence: |
ChIP |
| Target gene experiment description: |
The phenobarbital-responsive Enhancer module of UGT1A1 (gtPBREM)-driven Luciferaseplasmid (gtPBREM-Luc.) containing a 290-bp distal Enhancer module (23570/23180) was constructed based on pGL3-promoter (Promega). |
About TF
| TF name : |
CCAR1(CCAR1) |
| TF experiment: |
ChIP |
| TF experiment description: |
CCAR1 regulates CAR-mediated mRNA expression (Figs. 4 and 5) and reporter activity (Figs. 2 and 3) of UGT1A1 but not CYP2B6.We speculated that CCAR1 is differentially recruited to CAR-responsive Enhancer regions. To investigate this hypothesis,we carried out ChIP experiments in HepTR/CAR cells. |
About Function
| Enhancer function : |
CCAR1 enhances transcriptional activity of CAR and acts as an Enhancer-dependent selective cofactor of CAR for UGT1A1 expression. |
| Enhancer function experiment: |
Luciferase Reporter Assay |
Enhancer function
experiment description: |
The phenobarbital-responsive Enhancer module of UGT1A1 (gtPBREM)-driven luciferase reporter plasmid (gtPBREM-Luc.) containing a 290-bp distal Enhancer module (-23570/-23180) was constructed based on pGL3-promoter (Promega). |
About SNP
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene
Expression of target genes for the
enhancer
Enhancer associated SNPs