About Enhancer
| Enhancer ID: | E_02_0757 |
| Species: | human |
| Position : | chr3:150450617-150456921   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Breast cancer |
| Pubmed ID: | 29628309 |
| Enhancer experiment: | ChIP-seq,ChIP-qPCR |
| Enhancer experiment description: | We next assessed whether those estrogen-induced JMJD6 binding sites shared enhancer characteristics, including highly enriched H3K4me1/2 but low levels of H3K4me3. Heatmap and tag density plots confirmed they were, indeed, ERa-bound enhancers . Furthermore, it was reported recently that a group of enhancers, namely active enhancers, were essential for the transcriptional activation of estrogeninduced coding target genes |
About Target gene
| Target gene : | SIAH2(hSiah2),SIAH2(hSiah2),SIAH2(hSiah2) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-qPCR,qRT-PCR |
| Target gene experiment description: | To validate JMJD6 effects on eRNA production, total RNA extracted from MCF7 cells transfected with control siRNA or two independent siRNAs pecifically against JMJD6 in the presence or absence of strogen treatment were subjected to qRT-PCR analysis using primers specifically targeting several ERa and JMJD6 co-bound active enhancers nearby estrogen-induced coding genes, such as FOXC1, SIAH2, GREB1, NRIP1, and SMAD7.;To validate JMJD6 effects on eRNA production, total RNA extracted from MCF7 cells transfected with control siRNA or two independent siRNAs pecifically against JMJD6 in the presence or absence of strogen treatment were subjected to qRT-PCR analysis using primers specifically targeting several ERa and JMJD6 co-bound active enhancers nearby estrogen-induced coding genes, such as FOXC1, SIAH2, GREB1, NRIP1, and SMAD7.;To validate JMJD6 effects on eRNA production, total RNA extracted from MCF7 cells transfected with control siRNA or two independent siRNAs pecifically against JMJD6 in the presence or absence of strogen treatment were subjected to qRT-PCR analysis using primers specifically targeting several ERa and JMJD6 co-bound active enhancers nearby estrogen-induced coding genes, such as FOXC1, SIAH2, GREB1, NRIP1, and SMAD7. |
About TF
| TF name : | JMJD6(PSR,PTDSR,PTDSR1)ESR1(ER,ESR,ESRA,ESTRR,Era,NR3A1)JMJD6(PSR,PTDSR,PTDSR1)ESR1(ER,ESR,ESRA,ESTRR,Era,NR3A1)JMJD6(PSR,PTDSR,PTDSR1)ESR1(ER,ESR,ESRA,ESTRR,Era,NR3A1) |
| TF experiment: | siRNA,RT-qPCR |
| TF experiment description: | To validate JMJD6 effects on eRNA production, total RNA extracted from MCF7 cells transfected with control siRNA or two independent siRNAs specifically against JMJD6 in the presence or absence of estrogen treatment were subjected to RT-qPCR analysis using primers specifically targeting several ER? and JMJD6 co-bound active enhancers nearby estrogen-induced coding genes.;To validate JMJD6 effects on eRNA production, total RNA extracted from MCF7 cells transfected with control siRNA or two independent siRNAs specifically against JMJD6 in the presence or absence of estrogen treatment were subjected to RT-qPCR analysis using primers specifically targeting several ER? and JMJD6 co-bound active enhancers nearby estrogen-induced coding genes.;To validate JMJD6 effects on eRNA production, total RNA extracted from MCF7 cells transfected with control siRNA or two independent siRNAs specifically against JMJD6 in the presence or absence of estrogen treatment were subjected to RT-qPCR analysis using primers specifically targeting several ER? and JMJD6 co-bound active enhancers nearby estrogen-induced coding genes. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene