About Enhancer
| Enhancer ID: | E_02_0759 |
| Species: | human |
| Position : | chr19:61151074-61161722   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | -- |
| Pubmed ID: | 29514092 |
| Enhancer experiment: | ChIP-seq |
| Enhancer experiment description: | Active enhancers shared by monocytes and DCs (but not neutrophils) and those specific for monocytes were primed and activated with similar kinetics(Figure 2A, left and center). H3K4me1 and H3K27ac ChIP-seq tag densities in these enhancer regions initially increased at the MDP stage.Notably, the expression of genes with primed but not active (often called poised) enhancers was barely induced in monocytes and DCs (Figure S2). We found that the kinetics of DC-specific active enhancers were slower(Figure 2A, right); |
About Target gene
| Target gene : | Csf2ra(CD116,Csfgmra,GM-CSFR,GM-CSF-Ra) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq,Transgenic mice,RT-qPCR |
| Target gene experiment description: | Representative genome browser images of monocyte- or DC-specific en_x0002_hancers, associated with the Bfsp1 and Ehf genes, respec_x0002_tively, are depicted in Figures 2B and 2C.Thus, monocyte_x0002_and/or DC-specific active enhancers are gradually established at progenitor stages prior to the expression of associated genes. |
About TF
| TF name : | Irf8(H-ICSBP,ICSBP,ICSBP1,IMD32A,IMD32B,IRF-8) |
| TF experiment: | ChIP-seq,Transgenic mice |
| TF experiment description: | Analysis of Enhancer landscapes in Irf8/ mononuclear phagocyte progenitors and ChIP-seq of IRF8 binding sites in wild-type (WT) cells uncovered a critical role for IRF8.Using an in vitro monocyte differentiation system, we additionally showed that IRF8 binds to promoter-distal enhancers and promotes H3K4me1 enrichment at its binding sites.Consistent with these data, the correlation coefficient of H3K4me1 ChIP-seq tag densities between WT MDPs and Irf8/ MDPs was lower than that of the microarray data. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Irf8 | GMCSF-mediated signaling events | pid | 38 |
| Irf8 | Interferon alpha/beta signaling | reactome | 67 |
| Irf8 | Interferon gamma signaling | reactome | 88 |
| Irf8 | Validated targets of C-MYC transcriptional repression | pid | 63 |
| Irf8 | Hs_Type_II_interferon_signaling_(IFNG)_WP619_71168 | wikipathways | 10 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene