About Enhancer

Enhancer ID: E_02_0760
Species: human
Position : chr4:84277627-84281723
Biosample name:
Experiment class : Low+High throughput
Enhancer type: Super-Enhancer
Disease: Gastric cancer
Pubmed ID:  29511351
Enhancer experiment: 3C,ChIP-seq,qPCR
Enhancer experiment description: Overlapping analysis of FANTOM5 expression atlas and chromatin interaction dataset derived from 4DGenome revealed one potential enhancer region (chr4:8427762784281723) adjacent to human HPSE promoter(chr4: 8425591584256935). Chromosome conformation capture (3C) and nucleic acid sequencing revealed the endogenous chromatin looping between HPSE promoter and super enhancer in AGS cells

About Target gene

Target gene : HPSE(HPA,HPA1,HPR1,HPSE1,HSE1),HPSE(HPA,HPA1,HPR1,HPSE1,HSE1)
Strong evidence: --
Less strong evidence: Luciferase Reporter Assay,qPCR
Target gene experiment description: Dual-luciferase assay revealed that this super enhancer increased the activity of HPSE promoter in gastric cancer cell lines SGC-7901 and AGS, independent of the orientation or location.;Dual-luciferase assay revealed that this super enhancer increased the activity of HPSE promoter in gastric cancer cell lines SGC-7901 and AGS, independent of the orientation or location.

About TF

TF name : EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)
TF experiment: ChIP-seq,qPCR
TF experiment description: ChIP and qPCR assay revealed that ectopic expression or knockdown of HPSE eRNA increased and decreased the enrichment of EGR1 on HPSE promoter,which was attenuated by knockdown or overexpression of hnRNPU or p300, respectively.;ChIP and qPCR assay revealed that ectopic expression or knockdown of HPSE eRNA increased and decreased the enrichment of EGR1 on HPSE promoter,which was attenuated by knockdown or overexpression of hnRNPU or p300, respectively.

About Function

Enhancer function : HPSE eRNA promotes cancer progression through driving chromatin looping and regulating hnRNPU/p300/EGR1/HPSE axis.
Enhancer function experiment: ChIP,qPCR
Enhancer function
experiment description:		 ChIP and qPCR assay revealed that knockdown or ectopic expression of hnRNPU prevented the increased or decreased binding of hnRNPU or p300 to super Enhancer region following stable ectopic expression or knockdown of HPSE eRNA

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
EGR1 AP-1 transcription factor network pid 71
EGR1 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
EGR1 Downstream signaling in nave CD8+ T cells pid 69
EGR1 ErbB1 downstream signaling pid 105
EGR1 Glucocorticoid receptor regulatory network pid 85
EGR1 Interferon alpha/beta signaling reactome 67
EGR1 Regulation of Androgen receptor activity pid 54
EGR1 Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met) pid 80
EGR1 Signaling events mediated by PRL pid 23
EGR1 TCR netpath 261
EGR1 Trk receptor signaling mediated by PI3K and PLC-gamma pid 36
EGR1 Trk receptor signaling mediated by the MAPK pathway pid 34
EGR1 Prion diseases kegg 34
EGR1 Hs_Thyroid_Stimulating_Hormone_(TSH)_signaling_pathway_WP2032_89823 wikipathways 28
EGR1 Hs_Serotonin_Receptor_4-6-7_and_NR3C_Signaling_WP734_74438 wikipathways 15
EGR1 Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
EGR1 AP-1 transcription factor network pid 71
EGR1 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
EGR1 Downstream signaling in nave CD8+ T cells pid 69
EGR1 ErbB1 downstream signaling pid 105
EGR1 Glucocorticoid receptor regulatory network pid 85
EGR1 Interferon alpha/beta signaling reactome 67
EGR1 Regulation of Androgen receptor activity pid 54
EGR1 Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met) pid 80
EGR1 Signaling events mediated by PRL pid 23
EGR1 TCR netpath 261
EGR1 Trk receptor signaling mediated by PI3K and PLC-gamma pid 36
EGR1 Trk receptor signaling mediated by the MAPK pathway pid 34
EGR1 Prion diseases kegg 34
EGR1 Hs_Thyroid_Stimulating_Hormone_(TSH)_signaling_pathway_WP2032_89823 wikipathways 28
EGR1 Hs_Serotonin_Receptor_4-6-7_and_NR3C_Signaling_WP734_74438 wikipathways 15
EGR1 Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs