About Enhancer
| Enhancer ID: | E_02_0760 |
| Species: | human |
| Position : | chr4:84277627-84281723   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | Gastric cancer |
| Pubmed ID: | 29511351 |
| Enhancer experiment: | 3C,ChIP-seq,qPCR |
| Enhancer experiment description: | Overlapping analysis of FANTOM5 expression atlas and chromatin interaction dataset derived from 4DGenome revealed one potential enhancer region (chr4:84277627�84281723) adjacent to human HPSE promoter(chr4: 84255915�84256935). Chromosome conformation capture (3C) and nucleic acid sequencing revealed the endogenous chromatin looping between HPSE promoter and super enhancer in AGS cells |
About Target gene
| Target gene : | HPSE(HPA,HPA1,HPR1,HPSE1,HSE1),HPSE(HPA,HPA1,HPR1,HPSE1,HSE1) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,qPCR |
| Target gene experiment description: | Dual-luciferase assay revealed that this super enhancer increased the activity of HPSE promoter in gastric cancer cell lines SGC-7901 and AGS, independent of the orientation or location.;Dual-luciferase assay revealed that this super enhancer increased the activity of HPSE promoter in gastric cancer cell lines SGC-7901 and AGS, independent of the orientation or location. |
About TF
| TF name : | EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225) |
| TF experiment: | ChIP-seq,qPCR |
| TF experiment description: | ChIP and qPCR assay revealed that ectopic expression or knockdown of HPSE eRNA increased and decreased the enrichment of EGR1 on HPSE promoter,which was attenuated by knockdown or overexpression of hnRNPU or p300, respectively.;ChIP and qPCR assay revealed that ectopic expression or knockdown of HPSE eRNA increased and decreased the enrichment of EGR1 on HPSE promoter,which was attenuated by knockdown or overexpression of hnRNPU or p300, respectively. |
About Function
| Enhancer function : | HPSE eRNA promotes cancer progression through driving chromatin looping and regulating hnRNPU/p300/EGR1/HPSE axis. |
| Enhancer function experiment: | ChIP,qPCR |
| Enhancer function experiment description: |
ChIP and qPCR assay revealed that knockdown or ectopic expression of hnRNPU prevented the increased or decreased binding of hnRNPU or p300 to super Enhancer region following stable ectopic expression or knockdown of HPSE eRNA |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene