About Enhancer
| Enhancer ID: | E_02_0761 |
| Species: | human |
| Position : | chr15:66074477-66098025   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | -- |
| Pubmed ID: | 29507293 |
| Enhancer experiment: | Hi-C,ChIP-seq |
| Enhancer experiment description: | Finally, if an enhancer element within hierarchical SEs overlaps with a bin associated with a z-score greater than the threshold H-score, the element is referred to a hub enhancer, whereas the remaining enhancers at the same SE are termed non-hub enhancers |
About Target gene
| Target gene : | MYO1D(PPP1R108,myr4),SMYD3 (KMT3E,ZMYND1,ZNFN3A1,bA74P14.1) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | -- |
| Target gene experiment description: | we employed CRISPR-Cas9-mediated genome engineering to delete individual hub or non-hub enhancers with paired sgRNAs flanking the enhancer elements at the MYO1D SE.We observed that 3 of the 5 genes within the SE-containing TAD domain MYO1D,TMEM98 and SPACA3)displayed significant downregulation in mRNA expression, whereas the other two genes (PSMD11 and CDK5R1) remained unaffected , suggesting that the MYO1D SE may regulate only a subset of genes within the same TAD domain. |
About TF
| TF name : | GATA1(ERYF1,GATA-1,GF-1,GF1,NF-E1,NFE1,XLANP,XLTDA,XLTT)TAL1(SCL,TCL5,bHLHa17,tal-1)PAX5(ALL3,BSAP)EBF1(COE1,EBF,O/E-1,OLF1) |
| TF experiment: | ChIP-seq |
| TF experiment description: | Hub Enhancers contain significantly higher ChIP-seq binding signals for lineage-regulating master regulators than non-hub Enhancers, such as GATA1 and TAL1 in K562 cells, and PAX5 and EBF1 in GM12878 cells. |
About Function
| Enhancer function : | Hub enhancers are the major constituents responsible for SE functional and structural organization. |
| Enhancer function experiment: | ChIP-seq,CRISPR/Cas9 |
| Enhancer function experiment description: |
The signals for H3K27ac and DNase I hypersensitivity are slightly higher at hub than other types of Enhancers. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| GATA1 | C-MYB transcription factor network | pid | 87 |
| GATA1 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| GATA1 | IL3 | netpath | 84 |
| GATA1 | Notch-mediated HES/HEY network | pid | 48 |
| GATA1 | p73 transcription factor network | pid | 80 |
| GATA1 | Signaling events mediated by HDAC Class I | pid | 89 |
| GATA1 | Signaling events mediated by HDAC Class II | pid | 58 |
| PAX5 | C-MYB transcription factor network | pid | 87 |
| PAX5 | ID | netpath | 38 |
| EBF1 | Transcriptional regulation of white adipocyte differentiation | reactome | 79 |
| EBF1 | Hs_Tryptophan_metabolism_WP465_85055 | wikipathways | 45 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene