About Enhancer
| Enhancer ID: | E_02_0764 |
| Species: | human |
| Position : | chr3:69863244-70187654   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | Melanoma |
| Pubmed ID: | 29408204 |
| Enhancer experiment: | ChIP-seq,Western blot,siRNA,qPCR |
| Enhancer experiment description: | H3K27ac chromatin immunoprecipitation sequencing (ChIP-seq) profiling, which marks active enhancers, indicated the presence of SEs at the MITF gene locus in MITF-hi cells and substantially less enhancer signal at the same location in an MITF-lo cell line (LOXIMVI) and a normal human dermal fibroblast line. |
About Target gene
| Target gene : | MITF(CMM8,COMMAD,MI,WS2,WS2A,bHLHe32) |
| Strong evidence: | -- |
| Less strong evidence: | qPCR,ChIP-seq |
| Target gene experiment description: | To identify a set of MITF high- and low-expressing cells, we performed quantitative real-time reverse transcriptase-PCR analysis on 18 melanoma lines and found 8 lines with high MITF (MITF-hi) (>2-fold mean normalized MITF levels) and 5 lines with low MITF (MITF-lo) ( |
About TF
| TF name : | CDK7(CAK,CAK1,CDKN7,HCAK,MO15,STK1,p39MO15) |
| TF experiment: | siRNA |
| TF experiment description: | To verify that CDK7 blockade can lead to growth suppression, we subjected SK-MEL-5, SK-MEL-30, and UACC257 cells to small interfering RNA (siRNA) against CDK7 and observed consistent decreases in cell growth (see Supplementary Figure S6 online) compared with siRNA nontargeting controls. Cell lines with lower MITF levels (LOXIMVI, A375, SK-MEL-63) (see Supplementary Figure S7 online) were also sensitive to THZ1, exhibited appropriate CDK7 targeting, and showed an apoptotic response when exposed to the agent. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene