About Enhancer
| Enhancer ID: | E_02_0765 |
| Species: | human |
| Position : | chrx:106959618-106962917   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 29385519 |
| Enhancer experiment: | ChIP-qPCR,4C |
| Enhancer experiment description: | However, in contrast to the effects on the hormone-induced levels, these effects do not consistently correlate with deletions at the GILZ GBS14 enhancer and might reflect difficulties to quantify these transcripts by qPCR due to low expression levels in the absence of hormone treatment.To test if deletion of an individual GBS influences GR occupancy at the edited enhancer, we analyzed GR binding by ChIP-qPCR in a representative clonal cell line in which we deleted the GILZ GBS1. |
About Target gene
| Target gene : | TSC22D3(DIP,DSIPI,GILZ,TSC-22R) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | -- |
| Target gene experiment description: | To experimentally test the contribution of the smallest regulatory units of gene regulation, individual GR binding sequences (GBSs), we used the CRISPR/Cas9-system (25) to delete selected GBSs in their endogenous genomic context. We chose a GBS located 1.5 kb upstream of the GR target gene GILZ (glucocorticoid induced leucine zipper, alias TSC22D3) and one GBS 1.5 kb upstream of the target gene DUSP1. |
About TF
| TF name : | NR3C1(GCCR,GCR,GCRST,GR,GRL) |
| TF experiment: | ChIP-seq |
| TF experiment description: | To study the global connection between GR binding and GR-dependent gene regulation, we combined data from genome-wide GR binding experiments (Chromatin Immunoprecipitation followed by sequencing (ChIP-seq)) with RNA-seq data regarding gene expression changes upon GR-activation in A549 cells (3). |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene