About Enhancer
| Enhancer ID: | E_02_0766 |
| Species: | human |
| Position : | chr4:174441057-174442400   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Neuroblastoma |
| Pubmed ID: | 29379199 |
| Enhancer experiment: | ChIP-seq,Western blot |
| Enhancer experiment description: | We observe that two hours after shutdown, MYCN is significantly depleted from promoters and enhancers (Fig. 1f; Supplementary Fig. 2c,e,f) - a result revealed by ChIP-Rx cell-count normalization.Although both traditional ChIP-seq and ChIP-Rx reveal global loss of MYCN at promoters and enhancers, we do observe global loss of additional marks (H3K4me3, CTCF, H3K27ac, and RNA Pol II) at 24 hours post MYCN shutdown only in ChIP-Rx data. In contrast, induction of MYCN in parental SHEP neuroblastoma cells (Fig. 1c,d) results in loading of MYCN at active promoters and enhancers, and is coincident with increases in global H3K27ac. |
About Target gene
| Target gene : | HAND2(DHAND2,Hed,Thing2,bHLHa26,dHand),HAND2(DHAND2,Hed,Thing2,bHLHa26,dHand) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq |
| Target gene experiment description: | ChIP-seq tracks of MYCN and H3K27ac at 0, 2 and 6hrs post MYCN induction at RPL22, HAND2 and the upstream ID2 enhancer.Visual inspection of MYCN load at highly occupied genes revealed a diversity of binding profiles with some genes exhibiting promoter MYCN binding (RPL22) and others a more mixed array of promoter and enhancer binding (HAND2 & ID2). ;ChIP-seq tracks of MYCN and H3K27ac at 0, 2 and 6hrs post MYCN induction at RPL22, HAND2 and the upstream ID2 enhancer.Visual inspection of MYCN load at highly occupied genes revealed a diversity of binding profiles with some genes exhibiting promoter MYCN binding (RPL22) and others a more mixed array of promoter and enhancer binding (HAND2 & ID2). |
About TF
| TF name : | MYCN(MODED,N-myc,NMYC,ODED,bHLHe37)MYCN(MODED,N-myc,NMYC,ODED,bHLHe37) |
| TF experiment: | Western blot,ChIP-seq |
| TF experiment description: | Across MYCN-amplified neuroblastoma lines, we used chromatin immunoprecipitation coupled to high-throughput sequencing (ChIP-seq) to generate a consensus genome-wide map of ~10,000 regions that exhibit strong and consistent MYCN occupancy.;Across MYCN-amplified neuroblastoma lines, we used chromatin immunoprecipitation coupled to high-throughput sequencing (ChIP-seq) to generate a consensus genome-wide map of ~10,000 regions that exhibit strong and consistent MYCN occupancy. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| MYCN | Hs_Pathways_Affected_in_Adenoid_Cystic_Carcinoma_WP3651_89271 | wikipathways | 27 |
| MYCN | Hs_Pathways_Affected_in_Adenoid_Cystic_Carcinoma_WP3651_89271 | wikipathways | 27 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene