About Enhancer
| Enhancer ID: | E_02_0768 |
| Species: | human |
| Position : | chr14:56245000-56285000   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 29348663 |
| Enhancer experiment: | ChIP-seq,qPCR,Hi-C |
| Enhancer experiment description: | To test this possibility, we first attempted to identify the promoter/enhancer region of LEENE. In evaluating the genomic region surrounding LEENE, i.e., 20?kb up- and down-stream of the transcription start site (TSS) on chr14: 56,245,00056,285,000, we observed the enrichments of H3K27ac and H3K4me1 in the HUVEC chromatin immunoprecipitation (ChIP)-seq data available in the ENCODE Database, indicating an enhancer state of this DNA region.We did find that PS led to significant increase in the H3K27ac in the LEENE region as measured by ChIP-qPCR, indicating the activation of LEENE as an enhancer in ECs subjected to PS vs. |
About Target gene
| Target gene : | LINC00520(C14orf34),LINC00520(C14orf34),LINC00520(C14orf34) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-qPCR,qPCR,Hi-C |
| Target gene experiment description: | As shown in Fig. 1f, quantitative PCR (qPCR) with LEENE RNA-specific primers also revealed the significantly higher level of LEENE in ECs subjected to PS than to OS. To confirm the flow regulation of LEENE in ECs and explore its relevance to endothelial function, we tested whether LEENE is differentially regulated by tumor necrosis factor alpha (TNF?), which exerts pro-inflammatory effects similar to OS, and atorvastatin (ATV), which confers endothelial protective effects similar to PS. Resembling the opposite effects of OS and PS, TNF? decreased, while ATV increased the level of LEENE.These findings are in line with the differential levels of KLF2/KLF4-eNOS signaling.;As shown in Fig. 1f, quantitative PCR (qPCR) with LEENE RNA-specific primers also revealed the significantly higher level of LEENE in ECs subjected to PS than to OS. To confirm the flow regulation of LEENE in ECs and explore its relevance to endothelial function, we tested whether LEENE is differentially regulated by tumor necrosis factor alpha (TNF?), which exerts pro-inflammatory effects similar to OS, and atorvastatin (ATV), which confers endothelial protective effects similar to PS. Resembling the opposite effects of OS and PS, TNF? decreased, while ATV increased the level of LEENE.These findings are in line with the differential levels of KLF2/KLF4-eNOS signaling.;As shown in Fig. 1f, quantitative PCR (qPCR) with LEENE RNA-specific primers also revealed the significantly higher level of LEENE in ECs subjected to PS than to OS. To confirm the flow regulation of LEENE in ECs and explore its relevance to endothelial function, we tested whether LEENE is differentially regulated by tumor necrosis factor alpha (TNF?), which exerts pro-inflammatory effects similar to OS, and atorvastatin (ATV), which confers endothelial protective effects similar to PS. Resembling the opposite effects of OS and PS, TNF? decreased, while ATV increased the level of LEENE.These findings are in line with the differential levels of KLF2/KLF4-eNOS signaling. |
About TF
| TF name : | KLF2(LKLF)KLF4(EZF,GKLF)KLF2(LKLF)KLF4(EZF,GKLF)KLF2(LKLF)KLF4(EZF,GKLF) |
| TF experiment: | ChIP-qPCR |
| TF experiment description: | To confirm the association of such TFs on the promoter of LEENE, we performed ChIP-qPCR, which detected a robust binding between KLF4 and multiple regions within the promoter region of LEENE (marked by H3K4me3 peaks, Fig. 2a); these interactions were significantly increased by Ad-KLF4, which mimics the effect of PS and ATV.;To confirm the association of such TFs on the promoter of LEENE, we performed ChIP-qPCR, which detected a robust binding between KLF4 and multiple regions within the promoter region of LEENE (marked by H3K4me3 peaks, Fig. 2a); these interactions were significantly increased by Ad-KLF4, which mimics the effect of PS and ATV.;To confirm the association of such TFs on the promoter of LEENE, we performed ChIP-qPCR, which detected a robust binding between KLF4 and multiple regions within the promoter region of LEENE (marked by H3K4me3 peaks, Fig. 2a); these interactions were significantly increased by Ad-KLF4, which mimics the effect of PS and ATV. |
About Function
| Enhancer function : | LEENE-associated Enhancer plays a role in positive regulation of eNOS transcription |
| Enhancer function experiment: | CRISPR/Cas9 |
| Enhancer function experiment description: |
As a result of the Enhancer ablation, the transcription of LEENE and eNOS was significantly suppressed, in both DMSO (a control vehicle) and ATV-treated ECs |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene