About Enhancer
| Enhancer ID: | E_02_0769 |
| Species: | human |
| Position : | chr10:63525013-63527013   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | T-cell acute lymphoblastic leukemia |
| Pubmed ID: | 29326336 |
| Enhancer experiment: | ChIA-PET,CRISPR/Cas9 |
| Enhancer experiment description: | A ChIP-seq analysis of the ARID5B protein showed that ARID5B binds directly to the NOTCH-driven MYC enhancer (N-Me), which has been reported previously to be activated by NOTCH1 in T-ALL cells.Analysis of transcriptional activity using a luciferase reporter assay with the N-Me sequence also demonstrated that ARID5B knockdown significantly inhibited the enhancer activity.Hence, these results indicated that ARID5B directly activates the expression of MYC in T-ALL. |
About Target gene
| Target gene : | ARID5B(DESRT,MRF-2,MRF2) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | ChIA-PET |
| Target gene experiment description: | Importantly, a chromatinchromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the cohesin protein demonstrated a cis-regulatory interaction between the ?135-kb element and the ARID5B transcriptional start site (TSS).In fact, genetic knockout of the ?135-kb element by the CRISPR/Cas9 method resulted in a marked down-regulation of ARID5B gene expression.Thus, these results indicate that the TAL1-bound region at ?135 kb is the enhancer controlling ARID5B expression in T-ALL cells. |
About TF
| TF name : | TAL1(SCL,TCL5,bHLHa17,tal-1) |
| TF experiment: | CRISPR/Cas9 |
| TF experiment description: | In fact, genetic knockout of the ?135- kb element by the CRISPR/Cas9 method resulted in a marked down-regulation of ARID5B gene expression (Fig. 1G; Supplemental Fig. S1BE). Thus, these results indicate that the TAL1-bound region at ?135 kb is the enhancer controlling ARID5B expression in T-ALL cells. |
About Function
| Enhancer function : | The TAL1-bound region at ?135 kb is the Enhancer controlling ARID5B expression in T-ALL cells. |
| Enhancer function experiment: | CRISPR/Cas9 |
| Enhancer function experiment description: |
In fact, genetic knockout of the -135-kb element by the CRISPR/Cas9 method resulted in a marked down-regulation of ARID5B gene expression |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene