About Enhancer
| Enhancer ID: | E_02_0770 |
| Species: | mouse |
| Position : | chr7:143459172-143459787   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Malignancies |
| Pubmed ID: | 29284139 |
| Enhancer experiment: | Luciferase Reporter Assay |
| Enhancer experiment description: | As shown in Fig 5A, enh1 is in the second intron of Cdkn1c while enh2 is located in the 3 UTR. Luciferase reporter constructs were transfected into iMEFs stably overexpressing FOXD1 or a negative control vector. |
About Target gene
| Target gene : | Cdkn1c(AL024410,CDKI,Kip2,p57(kip2),p57Kip2),Cdkn1c(AL024410,CDKI,Kip2,p57(kip2),p57Kip2) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | Luciferase Reporter Assay,RT-PCR |
| Target gene experiment description: | We tested whether FOXD1 directly binds these enhancer sequences using established luciferase reporter constructs. As shown in Fig. 5A, enh1 is in the second intron of Cdkn1c while enh2 is located in the 3? UTR. Luciferase reporter constructs were transfected into iMEFs stably overexpressing FOXD1 or a negative control vector. FOXD1-expressing cells exhibited higher activity of enh1-driven luciferase than negative controls while no difference was observed in luciferase activity driven by enh2(Fig. 5B).;We tested whether FOXD1 directly binds these enhancer sequences using established luciferase reporter constructs. As shown in Fig. 5A, enh1 is in the second intron of Cdkn1c while enh2 is located in the 3? UTR. Luciferase reporter constructs were transfected into iMEFs stably overexpressing FOXD1 or a negative control vector. FOXD1-expressing cells exhibited higher activity of enh1-driven luciferase than negative controls while no difference was observed in luciferase activity driven by enh2(Fig. 5B). |
About TF
| TF name : | Foxd1(AI385632,BF-2,FREAC4,Hfh10,Hfhbf2)Foxd1(AI385632,BF-2,FREAC4,Hfh10,Hfhbf2) |
| TF experiment: | Luciferase Reporter Assay |
| TF experiment description: | Firefly luciferase reporter assay driven by Cdkn1c enhancer elements, enh1 and enh2, in iMEFs overexpressing empty pLenti construct or FOXD1.;Firefly luciferase reporter assay driven by Cdkn1c enhancer elements, enh1 and enh2, in iMEFs overexpressing empty pLenti construct or FOXD1. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene