About Enhancer
| Enhancer ID: | E_02_0771 |
| Species: | mouse |
| Position : | chr2:126601468-126603468   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Ige/mast cell-mediated anaphylaxis |
| Pubmed ID: | 29277702 |
| Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay |
| Enhancer experiment description: | Chromatin immunoprecipitation sequencing analysis identified putative Hdc enhancers.Luciferase reporter transcription assay confirmed enhancer activities of putative enhancers in the Hdc gene.MITF bound to an enhancer located 8.8 kb upstream of the transcription start site of the Hdc gene and directed enhancer activity.To determine whether the putative Hdc Enhancers function in Hdc gene transcription, we constructed a luciferase reporter gene in which luciferase expression is under the control of the Hdc minimal promoter (-44 to +1 relative to the TSS) and the -8.8 or +0.3 Hdc Enhancer. We found that the -8.8 Hdc Enhancer dramatically increased the activity of the Hdc minimal promoter (13.4-fold increase) , whereas the +0.3 Hdc Enhancer failed to show significant Enhancer activity. |
About Target gene
| Target gene : | Hdc(AW108189-a,Hdc-c,Hdc-e,Hdc-s,Hdc),Hdc(AW108189-a,Hdc-c,Hdc-e,Hdc-s,Hdc),Hdc(AW108189-a,Hdc-c,Hdc-e,Hdc-s,Hdc) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-qPCR,Luciferase Reporter Assay |
| Target gene experiment description: | Chromatin immunoprecipitation sequencing analysis identified putative Hdc enhancers.Luciferase reporter transcription assay confirmed enhancer activities of putative enhancers in the Hdc gene.MITF bound to an enhancer located 8.8 kb upstream of the transcription start site of the Hdc gene and directed enhancer activity.;Chromatin immunoprecipitation sequencing analysis identified putative Hdc enhancers.Luciferase reporter transcription assay confirmed enhancer activities of putative enhancers in the Hdc gene.MITF bound to an enhancer located 8.8 kb upstream of the transcription start site of the Hdc gene and directed enhancer activity.;Chromatin immunoprecipitation sequencing analysis identified putative Hdc enhancers.Luciferase reporter transcription assay confirmed enhancer activities of putative enhancers in the Hdc gene.MITF bound to an enhancer located 8.8 kb upstream of the transcription start site of the Hdc gene and directed enhancer activity. |
About TF
| TF name : | Gata2(DCML,IMD21,MONOMAC,NFE1B)Mitf(CMM8,COMMAD,MI,WS2,WS2A,bHLHe32)Gata2(DCML,IMD21,MONOMAC,NFE1B)Mitf(CMM8,COMMAD,MI,WS2,WS2A,bHLHe32)Gata2(DCML,IMD21,MONOMAC,NFE1B)Mitf(CMM8,COMMAD,MI,WS2,WS2A,bHLHe32) |
| TF experiment: | ChIP-qPCR,shRNA,Luciferase Reporter Assay |
| TF experiment description: | Our ChIP-qPCR analysis of MITF binding in BMMCs revealed that among five predicted MITF-binding sites located within the -8.8 and +0.3 Hdc Enhancers.We found that Hdc mRNA expression was significantly diminished (70% reduction) in TBMMCs with knocked down Mitf mRNA expression.Conversely, overexpression of MITF in a non-mast cell line greatly 383 increased the activity of the -8.8 Hdc Enhancer.;Our ChIP-qPCR analysis of MITF binding in BMMCs revealed that among five predicted MITF-binding sites located within the -8.8 and +0.3 Hdc Enhancers.We found that Hdc mRNA expression was significantly diminished (70% reduction) in TBMMCs with knocked down Mitf mRNA expression.Conversely, overexpression of MITF in a non-mast cell line greatly 383 increased the activity of the -8.8 Hdc Enhancer.;Our ChIP-qPCR analysis of MITF binding in BMMCs revealed that among five predicted MITF-binding sites located within the -8.8 and +0.3 Hdc Enhancers.We found that Hdc mRNA expression was significantly diminished (70% reduction) in TBMMCs with knocked down Mitf mRNA expression.Conversely, overexpression of MITF in a non-mast cell line greatly 383 increased the activity of the -8.8 Hdc Enhancer. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene